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Identification of T‐cell epitopes from benzylpenicillin conjugated to human serum albumin and implication in penicillin allergy

机译：鉴别人血清白蛋白与人血清白蛋白缀合的T细胞表位和青霉素过敏的影响

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Abstract Background There is in vitro evidence that T cells from allergic patients react to benzylpenicillin‐human serum albumin ( BP ‐ HSA ) bioconjugates. Our group has recently shown the existence of na?ve CD 4 + T cells recognizing BP ‐ HSA in healthy donors. However, BP ‐haptenated peptides from HSA participating in the immunization of allergic patients have never been identified. The purpose of the present study is to identify immunodominant BP ‐haptenated peptides from HSA involved in immunization of patients to BP and to refine the frequency calculation of na?ve CD 4 + T cells recognizing BP . Methods Co‐cultures were established with CD 4 + T cells from non‐allergic donors and mature autologous dendritic cells ( DC s) loaded with BP ‐ HSA or BP ‐haptenated peptides from HSA . The CD 4 + T‐cell response specific for BP ‐ HSA or for individual BP ‐haptenated peptides was measured using an interferon‐γ ( IFN ‐γ) ELIS pot assay. The frequency of BP ‐specific CD 4 + T cells was then calculated using the Poisson distribution. BP ‐ HSA and BP ‐haptenated peptides recognition by allergic patients was evaluated on peripheral blood mononuclear cells ( PBMC s) using a lymphocyte transformation test ( LTT ). Results Results showed that BP ‐ HSA and BP ‐haptenated peptides were recognized by na?ve T cells from 15/16 and 13/14 tested healthy donors, respectively. Most donors responded to 3 peptides with BP covalently bound on lysines 159, 212, and 525. Two of these benzylpenicilloylated peptides (lysines 159 and 525) were also found to induce PBMC s proliferation in patients with allergic reaction to penicillins. Conclusion This study identifies and characterizes for the first time the BP ‐haptenated peptides from HSA involved in the immunization of patients to penicillins.
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  • 来源
    《Allergy》 |2018年第8期|共11页
  • 作者

    Azoury M. E.; Filì L.; Bechara R.; Scornet N.; Chaisemartin L.; Weaver R. J.; Claude N.; Maillere B.; Parronchi P.; Joseph D.; Pallardy M.;

  • 作者单位

    Inflammation Chimiokines et ImmunopathologieUniversité Paris‐SaclayChatenay‐Malabry France;

    Department of Experimental and Clinical MedicineUniversity of FlorenceFlorence Italy;

    Inflammation Chimiokines et ImmunopathologieUniversité Paris‐SaclayChatenay‐Malabry France;

    BioCISUniversité Paris‐SaclayChatenay‐Malabry France;

    Inflammation Chimiokines et ImmunopathologieUniversité Paris‐SaclayChatenay‐Malabry France;

    Institut de Recherches Internationales ServierSuresnes France;

    Institut de Recherches Internationales ServierSuresnes France;

    SIMOPROCEASaclay France;

    Department of Experimental and Clinical MedicineUniversity of FlorenceFlorence Italy;

    BioCISUniversité Paris‐SaclayChatenay‐Malabry France;

    Inflammation Chimiokines et ImmunopathologieUniversité Paris‐SaclayChatenay‐Malabry France;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学免疫学;
  • 关键词

    allergy; drug allergy; hapten; penicillin; T cell;

    机译：过敏;药物过敏;Hapten;青霉素;T细胞;

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