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首页> 外文期刊>ACS catalysis >Synthesis of gamma-Hydroxy-alpha-amino Acid Derivatives by Enzymatic Tandem Aldol Addition-Transamination Reactions
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Synthesis of gamma-Hydroxy-alpha-amino Acid Derivatives by Enzymatic Tandem Aldol Addition-Transamination Reactions

机译：通过酶促串联醛醇添加衍生反应合成γ-羟基-α-氨基酸衍生物

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摘要

Three enzymatic routes toward gamma-hydroxy-alpha-amino acids by tandem aldol addition-transamination one-pot two-step reactions are reported. The approaches feature an enantioselective aldol addition of pyruvate to various nonaromatic aldehydes catalyzed by trans-o-hydroxybenzylidene pyruvate hydratase-aldolase (HBPA) from Pseudomonas putida. This affords chiral 4-hydroxy-2-oxo acids, which were subsequently enantioselectively aminated using S-selective transaminases. Three transamination processes were investigated involving different amine donors and transaminases: (i) L-Ala as an amine donor with pyruvate recycling, (ii) a benzylamine donor using benzaldehyde lyase from Pseudomonas fluorescens Biovar I (BAL) to transform the benzaldehyde formed into benzoin, minimizing equilibrium limitations, and (iii) L-Glu as an amine donor with a double cascade comprising branched-chain alpha-amino acid aminotransferase (BCAT) and aspartate amino transferase (AspAT), both from E. coli, using L-Asp as a substrate to regenerate L-Glu. The gamma-hydroxy-alpha-amino acids thus obtained were transformed into chiral alpha-amino-gamma-butyrolactones, structural motifs found in many biologically active compounds and valuable intermediates for the synthesis of pharmaceutical agents.
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