Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein

Luyan  Yang; Tian  Gao; Wenjing  Li; Yu  Luo; Salim  Ullah; Xiaona  Fang; Yanwei  Cao; Renjun  Pei

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Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein

机译：Ni-硝基乙酸亲和力选择方法，用于选择N-Cadherin蛋白特异的DNA适体

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摘要

Nucleic acid aptamers are single-stranded oligonucleotides that may be evolved for affinity and specificity for their targets and can be easily produced, regenerated, and stabilized. In this study, we adapted Ni-NTA (nickle-charged nitrilotriacetic acid) affinity-chromatography in the development of single-stranded DNA aptamers against N-cadherin protein by systematic evolution of ligands by exponential enrichment (SELEX). After ten rounds of selection, two aptamers, designated NS13 and NC23, were selected, which showed low dissociation constants of 93 and 174 nM, respectively. The 5′-carboxyfluorescein-labeled NS13 was used for the sensitive detection of N-cadherin protein by the enzyme-linked oligonucleotide assay (ELONA) method.

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来源
《ACS combinatorial science》 |2020年第12期|共6页
作者
Luyan Yang; Tian Gao; Wenjing Li; Yu Luo; Salim Ullah; Xiaona Fang; Yanwei Cao; Renjun Pei;
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作者单位

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences;

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原文格式 PDF
正文语种 eng
中图分类化学;
关键词
N-cadherin; SELEX; nucleic acid aptamer; enzyme-linked oligonucleotide assay;

机译：n-cadherin;selex;核酸适体;酶联寡核苷酸测定;

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2. Evolution of specific RNA aptamers via SELEX targeting recombinant human CD36 protein: A candidate therapeutic target in severe malaria [J] . Nik Abdul Aziz Nik Kamarudin, Judy Nur Aisha Sat, Nur Fatihah Mohd Zaidi, 亚太热带生物医学杂志（英文版） . 2020,第001期
3. Isolation of Specific sncRNA Binding Proteins Using the Streptomycin Binding RNA Aptamer as an Affinity Tag [J] . NikolaiWINDBICHLER, RupertGROfβBERGER, ReneeSCHROEDER 生物化学与生物物理学报：英文版 . 2004,第002期
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Ni-Nitrilotriacetic Acid Affinity SELEX Method for Selection of DNA Aptamers Specific to the N-Cadherin Protein

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