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首页> 外文期刊>Animal Science Journal >Non-invasive assessment of culture media from goat cloned embryos associated with subjective morphology by gas chromatography - mass spectroscopy-based metabolomic analysis
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Non-invasive assessment of culture media from goat cloned embryos associated with subjective morphology by gas chromatography - mass spectroscopy-based metabolomic analysis

机译：通过气相色谱 - 基于质谱基代代谢分析与山羊克隆胚胎的植物克隆胚胎的非侵入性评估

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摘要

Pre-implantation embryo metabolism demonstrates distinctive characteristics associated with the development potential of embryos. We aim to determine if metabolic differences correlate with embryo morphology. In this study, gas chromatography - mass spectroscopy (GC-MS)-based metabolomics was used to assess the culture media of goat cloned embryos collected from high-quality (HQ) and low-quality (LQ) groups based on morphology. Expression levels of amino acid transport genes were further examined by quantitative real-time PCR. Results showed that the HQ group presented higher percentages of blastocysts compared with the LQ counterparts (P & 0.05). Metabolic differences were also present between HQ and LQ groups. The culture media of the HQ group showed lower levels of valin, lysine, glutamine, mannose and acetol, and higher levels of glucose, phytosphingosine and phosphate than those of the LQ group. Additionally, expression levels of amino acid transport genes SLC1A5 and SLC3A2 were significantly lower in the HQ group than the LQ group (P & 0.05, respectively). To our knowledge, this is the first report which uses GC-MS to detect metabolic differences in goat cloned embryo culture media. The biochemical profiles may help to select the most in vitro viable embryos.
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  • 来源
    《Animal Science Journal》 |2018年第1期|共11页
  • 作者

    Zhang Yan-Li; Zhang Guo-Min; Jia Ruo-Xin; Wan Yong-Jie; Yang Hua; Sun Ling-Wei; Han Le; Wang Feng;

  • 作者单位

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ Coll Anim Sci Jiangsu Livestock Embryo Engn Lab 1 Weigang Nanjing 210095 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 畜牧、动物医学、狩猎、蚕、蜂;
  • 关键词

    cloned embryo; culture media; GC-MS; goat; metabolomics;

    机译：克隆胚胎;培养基;GC-MS;山羊;代谢组学;

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