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首页> 外文期刊>Archives of microbiology >Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu) (UAA) gene bldA
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Properties of Streptomyces albus J1074 mutant deficient in tRNA(Leu) (UAA) gene bldA

机译：TRNA（LEU）（UAA）基因BLDA中缺血性缺乏缺乏症的链霉菌的性质

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摘要

Streptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNA(Leu) (UAA), the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media. In addition, bldA mutant of J1074 was unable to produce endogenous antibacterial compounds and two heterologous antibiotics, moenomycin and aranciamycin, whose biosynthesis is directed by TTA-containing genes. We have employed a new TTA codon-specific beta-galactosidase reporter system to provide genetic evidence that J1074 bldA mutant is impaired in translation of TTA. In addition, we have discussed the possible reasons for differences in the phenotypes of bldA mutants described here and in previous studies, providing knowledge to study bldA-based regulation of antibiotic biosynthesis.
机译：None

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  • 来源
    《Archives of microbiology》 |2017年第8期|共9页
  • 作者

    Koshla Oksana; Lopatniuk Maria; Rokytskyy Ihor; Yushchuk Oleksandr; Dacyuk Yuriy; Fedorenko Victor; Luzhetskyy Andriy; Ostash Bohdan;

  • 作者单位

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Ivan Franko Natl Univ Lviv Dept Phys Earth 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

    Saarland Univ Helmholtz Inst Pharmaceut Res Saarland Dept Pharmaceut Biotechnol Actinobacteria;

    Ivan Franko Natl Univ Lviv Dept Genet &

    Biotechnol 4 Hrushevskoho St UA-79005 Lvov Ukraine;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

    Streptomyces albus; tRNA; Antibiotics; beta-galactosidase;

    机译：Streptomyces Albus;TRNA;抗生素;β-半乳糖苷酶;

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