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首页> 外文期刊>Archives of virology >Development and application of a novel triplex protein microarray method for rapid detection of antibodies against avian influenza virus, Newcastle disease virus, and avian infectious bronchitis virus
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Development and application of a novel triplex protein microarray method for rapid detection of antibodies against avian influenza virus, Newcastle disease virus, and avian infectious bronchitis virus

机译：一种新型三链蛋白微阵列方法的开发与应用，用于快速检测禽流感病毒，新城疫病毒和禽类传染性支气管炎病毒的抗体

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摘要

Avian influenza virus (AIV), Newcastle disease virus (NDV), and avian infectious bronchitis virus (IBV) inflict immense damage on the global poultry industry annually. Serological diagnostic methods are fundamental for the effective control and prevention of outbreaks caused by these viruses. In this study, a novel triplex protein microarray assay was developed and validated for the rapid and simultaneous visualized detection of antibodies against AIV, NDV, and IBV in chicken sera. The AIV nuclear protein (NP), NDV phosphoprotein (P), and IBV nonstructural protein 5 (nsp5) were produced in a prokaryotic expression system, purified, and immobilized onto an initiator integrated poly(dimethylsiloxane) (iPDMS) film as probes to detect antibodies against these viruses in chicken sera. After optimization of the reaction conditions, no cross-reactivity was detected with infectious bursal disease virus, avian leukosis virus subgroup J and chicken anemia virus antisera. The lowest detectable antibody titers in this assay corresponded to hemagglutination inhibition (HI) titers of 2(4) and 2(1) for AIV and NDV, respectively, and to an IDEXX antibody titer of 10(3) for IBV, using the HI assay and IDEXX commercial ELISA kit as the reference methods. When156 serum samples were tested using the new assay, the HI test and the IBV IDEXX ELISA kit, the assay showed 96.8% (151/156), 97.4% (152/156) and 99.4% (155/156) diagnostic accuracy for detection of AIV, NDV and IBV antibody, respectively. The current study suggests that the newly developed triplex microarray is rapid, sensitive, and specific, providing a viable alternative assay for AIV, NDV, and IBV antibody screening in epidemiological investigations and vaccination evaluations.
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  • 来源
    《Archives of virology》 |2021年第4期|共12页
  • 作者

    Li Yuan; Hu Jianhua; Lei Jing; Fan Wentao; Bi Zhenwei; Song Suquan; Yan Liping;

  • 作者单位

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

    Comprehens Enforcement Brigade Agr Forestry &

    Wat Suzhou 215000 Peoples R China;

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

    Nanjing Agr Univ MOE Joint Int Res Lab Anim Hlth &

    Food Safety Jiangsu Engn Lab Anim Immunol;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学微生物学（病原细菌学、病原微生物学）;
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