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首页> 外文期刊>International journal of biomedical nanoscience and nanotechnology >Enzymatically synthesised nano-gold decorated reduced graphene oxide for development of an enhanced glucose oxidase-based amperometric biosensor
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Enzymatically synthesised nano-gold decorated reduced graphene oxide for development of an enhanced glucose oxidase-based amperometric biosensor

机译：酶促合成的纳米金装饰石墨烯氧化物，用于显影增强的葡萄糖氧化酶的水平体传感器

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摘要

We report biological procedure of synthesising nano-gold (AuNPs) over reduced graphene oxide (rGO) using physiologically important enzyme β galactosidase. The Au@rGO NC was characterised using biophysical techniques. Au@rGO immobilised with glucose oxidase (GOx) was achieved by drop-coating glassy carbon electrode surface with Nation (Nf) as preservative membrane. EIS, amperometric response and cyclic voltammetry were used to determine electrochemical properties of the biosensor. The Nf/GOx/Au@rGO/GCE biosensor had sensitivity of 18.25μA mM~-1 cm~-2 and linear range of 0.05-18 mM (R2 = 0.997) with detection limit of 36 μM (S/N = 3). The response time over a period of 60 days depicted the biosensor to be quite stable. The potential of the bioelectrode in assessment of human blood glucose concentrations was compared with commercially available glucose sensors. The sensitivity and stability infer the biosensor is capable of functioning specifically in analysis of glucose in physiological fluids.
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