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首页> 外文期刊>General Physiology and Biophysics >The possible regulatory effect of the PD-1/PD-L1 signaling pathway on Tregs in ovarian cancer
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The possible regulatory effect of the PD-1/PD-L1 signaling pathway on Tregs in ovarian cancer

机译：PD-1 / PD-L1信号通路在卵巢癌中的Tregs上可能的调节作用

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Aim of this study was to investigate the possible regulatory effect of the programmed death-1 (PD-1)/programmed death ligand-1 (PD-L1) signaling pathway on Tregs in ovarian cancer. Immunohistochemistry was used to detect the expression of PD-L1 and PD-1 and the presence of FOXP3(+) Tregs in ovarian cancer. Then, ovarian cancer HO8910 cells were subjected to transfection with PD-L1 siRNA in vitro. CCK-8, Transwell and wound healing assays were performed to detect the biological behaviors of ovarian cancer cells. Human T- cells isolated from human peripheral blood were cocultured with HO8910 cells, which were divided into the Control, TGF-beta, and TGF-beta(+) anti-PD-L1 groups. The proportion of differentiated Tregs was detected by flow cytometry. Mouse models of ovarian cancer were established, and PD-L1 antibody therapy was administered. Tumor growth and Treg recruitment were observed. PD-L1, PD-1 and FOXP3(+) Tregs were found in ovarian cancer tissue. Patients with tumors with an advanced stage and low differentiation and lymph node metastasis had significantly higher levels of PD-1, PD-L1 and FOXP3(+) Tregs. After transfection with PD-L1 siRNA, HO8910 cells showed a significant reduction in PD-L1 expression, proliferation, migration and invasion. After T-cells were cocultured with ovarian cancer cells, the TGF-beta(+) anti-PD-Li group showed a substantial decline in the differentiation of T-cells into Tregs compared with the TGF-beta group. Moreover, mice in the anti-PD-L1 group had significantly reduced tumor growth rates, Treg proportions in the tumor microenvironment, and FOXP3 expression.
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