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首页> 外文期刊>Parasitology >Prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile
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Prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile

机译：Bartonella SPP的患病率，血液学发现和遗传多样性。 在Valdivia，智利南部的家养猫

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摘要

The present study determined the prevalence, hematological findings and genetic diversity of Bartonella spp. in domestic cats from Valdivia, Southern Chile. A complete blood count and nuoG gene real-time quantitative PCR (qPCR) for Bartonella spp. were performed in 370 blood samples from cats in Valdivia, Southern Chile. nuoG qPCR-positive samples were submitted to conventional PCR for the gltA gene and sequencing for species differentiation and phylogenetic analysis. Alignment of gltA gene was used to calculate the nucleotide diversity, polymorphic level, number of variable sites and average number of nucleotide differences. Bartonella DNA prevalence in cats was 18.1% (67/370). Twenty-nine samples were sequenced with 62.0% (18/29) identified as Bartonella henselae, 34.4% (10/29) as Bartonella clarridgeiae, and 3.4% (1/29) as Bartonella koehlerae. Bartonella-positive cats had low DNA bacterial loads and their hematological parameters varied minimally. Each Bartonella species from Chile clustered together and with other Bartonella spp. described in cats worldwide. Bartonella henselae and B. clarridgeiae showed a low number of variable sites, haplotypes and nucleotide diversity. Bartonella clarridgeiae and B. koehlerae are reported for the first time in cats from Chile and South America, respectively.
机译：本研究确定了巴尔通体属在智利南部瓦尔迪维亚家猫中的患病率、血液学发现和遗传多样性。对智利南部瓦尔迪维亚的370份猫血液样本进行了巴尔通体属的全血计数和nuoG基因实时定量PCR（qPCR）。将nuoG qPCR阳性样本提交给gltA基因的常规PCR，并进行物种分化和系统发育分析测序。gltA基因比对用于计算核苷酸多样性、多态性水平、可变位点数和核苷酸差异的平均数。猫巴尔通体DNA患病率为18.1%（67/370）。对29份样本进行测序，其中62.0%（18/29）的样本被鉴定为鸡巴尔通体，34.4%（10/29）的样本被鉴定为克拉氏巴尔通体，3.4%（1/29）的样本被鉴定为科勒巴尔通体。巴尔通体阳性猫的DNA细菌载量较低，血液学参数变化最小。来自智利的每一种巴尔通体都聚集在一起，并与世界各地猫科动物中描述的其他巴尔通体属聚集在一起。亨氏巴尔通体和克拉氏巴尔通体的变异位点、单倍型和核苷酸多样性均较低。首次在智利和南美洲的猫身上发现了克拉氏巴尔通体和库勒氏巴尔通体。

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