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The efficacy of recombinant protein lbk39 for the diagnosis of leishmaniosis in dogs

机译：重组蛋白LBK39对犬利施诊断的疗效

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Visceral leishmaniosis is one of the most important zoonotic diseases on the planet and dogs are the main reservoir of canine visceral leishmaniosis (CVL) in endemic areas. They play an important role in human infection because in dogs the disease appears long time after infection, and they can move uncontrollably, contributing to disperse the parasite. To take the decision to treat the animals or for euthanasia, in an elimination programme, in order to reduce the parasitic load, it is necessary to diagnose correctly, having more effective tools. Our group has developed a new recombinant antigen-based kinesin-related gene of Leishmania brazilien-sis (Lbk39), which shows 59% amino acid identity to the L. infantum homologue. The Lbk39 gene was synthesized, inserted into the pLEXSY-sat2 vector and transfected into L. tarentolae cells by electroporation. The recombinant protein was secreted in the culture with a C-ter-minal histidine marker, purified, generating a product at 337.68μgmL-1. A total of 152 sera from dog’s endemic and non-endemic areas were used, being 78 positives and 75 negatives. The antigen Lbk39 showed 100% sensitivity and 96.1% specificity. We compared this antigen with other antigens such as total extract of the parasite, TRDPP, and our data indicate that Lbk39 has potential application in the diagnosis of CVL through antibody detection.
机译：内脏利什曼病是地球上最重要的人畜共患疾病之一，犬是流行地区犬内脏利什曼病（CVL）的主要宿主。它们在人类感染中起着重要作用，因为在狗身上，这种疾病在感染后很长时间内就会出现，它们可以不受控制地移动，有助于寄生虫的传播。为了在消除计划中做出治疗动物或安乐死的决定，为了减少寄生虫负荷，有必要使用更有效的工具进行正确诊断。我们的团队已经开发了一种新的重组基于抗原的巴西利什曼原虫驱动蛋白相关基因（Lbk39），该基因与婴儿利什曼原虫同源物的氨基酸同源性为59%。合成Lbk39基因，将其插入pLEXSY-sat2载体，并通过电穿孔法将其转染到塔伦托乳杆菌细胞中。重组蛋白在培养基中用C-末端组氨酸标记物分泌，纯化后产生337.68μgmL-1的产物。共使用了152份来自狗的地方病和非地方病地区的血清，其中78份阳性，75份阴性。抗原Lbk39的敏感性为100%，特异性为96.1%。我们将该抗原与寄生虫总提取物TRDPP等其他抗原进行了比较，我们的数据表明，Lbk39在通过抗体检测诊断CVL方面具有潜在的应用价值。

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