Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

Corral M. A.; Paula F. M.; Meisel D. M. C. L.; Castilho V. L. P.; Goncalves E. M. N.; Levy D.; Bydlowski S. P.; Chieffi P. P.; Castro-Borges W.; Gryschek R. C. B.

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Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

机译：使用异源抗原诊断人抗原的潜在免疫标志物

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Strongyloides venezuelensis is a parasitic nematode of rodents that is frequently used to obtain heterologous antigens for immunological diagnosis of human strongyloidiasis. The aim of this study was to identify antigens from filariform larvae of S. venezuelensis for immunodiagnosis of human strongyloidiasis. Soluble and membrane fractions from filariform larvae of S. venezuelensis were obtained in phosphate saline (SS and SM) and in Tris-HCl buffer (TS and TM), and were analysed by Western blotting. Different antigenic components were recognized by IgG antibodies from the sera of strongyloidiasis patients. Highest recognition was observed for a 30-40 kDa mass range present in all antigenic fractions. The band encompassing this mass range was then excised and subjected to mass spectrometry for protein identification. Immunoreactive proteins identified in the soluble fractions corresponded to metabolic enzymes, whereas cytoskeletal proteins and galectins were more abundant in the membrane fractions. These results represent the first approach towards identification of S. venezuelensis antigens for use in immunodiagnostic assays for human strongyloidiasis.

机译：委内瑞拉圆线虫是一种啮齿动物寄生线虫，常用于获取异源抗原，用于人类圆线虫病的免疫学诊断。本研究的目的是从委内瑞拉链球菌丝状幼虫中鉴定抗原，用于人类圆线虫病的免疫诊断。在磷酸盐盐水（SS和SM）和Tris-HCl缓冲液（TS和TM）中获得了威尼斯丝状体幼虫的可溶性和膜组分，并通过Western印迹分析。从类圆线虫病患者血清中提取的IgG抗体可识别不同的抗原成分。在所有抗原组分的30-40 kDa质量范围内观察到最高识别率。然后切除包含该质量范围的条带，并进行质谱分析以进行蛋白质鉴定。可溶性组分中鉴定的免疫反应蛋白与代谢酶相对应，而细胞骨架蛋白和半乳糖凝集素在膜组分中更丰富。这些结果代表了第一种用于人类圆线虫病免疫诊断分析的委内瑞拉链球菌抗原鉴定方法。

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《Parasitology》 |2017年第2期|共7页
作者
Corral M. A.; Paula F. M.; Meisel D. M. C. L.; Castilho V. L. P.; Goncalves E. M. N.; Levy D.; Bydlowski S. P.; Chieffi P. P.; Castro-Borges W.; Gryschek R. C. B.;
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Univ Sao Paulo Fac Med Hosp Clin LIM 06 Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin LIM 06 Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin LIM 06 Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin Div Lab Cent Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin Div Lab Cent Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin LIM 31 Sao Paulo SP Brazil;

Univ Sao Paulo Fac Med Hosp Clin LIM 31 Sao Paulo SP Brazil;

Santa Casa Fac Ciencias Med Sao Paulo SP Brazil;

Univ Fed Ouro Preto Dept Ciencias Biol Ouro Preto MG Brazil;

Univ Sao Paulo Fac Med Hosp Clin LIM 06 Sao Paulo SP Brazil;

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原文格式 PDF
正文语种 eng
中图分类寄生虫病;
关键词
Strongyloides venezuelensis; immunodiagnostic; antigens; actin; galectin;

机译：酮乙烯基毒素;免疫诊断;抗原;actin;galectin;

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Potential immunological markers for diagnosis of human strongyloidiasis using heterologous antigens

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