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首页> 外文期刊>Parasitology >Evaluation of immune response to Bacillus subtilis spores expressing Clonorchis sinensis serpin3
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Evaluation of immune response to Bacillus subtilis spores expressing Clonorchis sinensis serpin3

机译：对表达Clonorchis Silpin3的枯草芽孢杆菌孢子的免疫应答评价

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摘要

Clonorchis sinensis (C. sinensis) is one of the most serious food-borne parasites, which can lead to liver fibrosis or cholangiocarcinoma. Effective measures for clonorchiasis prevention are still urgently needed. Bacillus subtilis (B. subtilis) is an effective antigen delivery platform for oral vaccines. Chonorchis sinensis serpin (CsSerpin) was proved to be potential vaccine candidates. In this study, CsSerpin3 was displayed on the surface of B. subtilis spore and recombinant spores were orally administrated to BALB/C mice. CsSerpin3-specific IgA levels in faecal, bile and intestinal mucous increased at 4-8 weeks after the first administration compared with those in control groups. The mucus production and the number of goblet cells in intestinal mucosa elevated in B.s-CotC-CsSerpin3 (CotC, coat protein of B. subtilis spore) spores treated group compared to those in blank control. No significant difference in the activities of glutamic-pyruvic transaminase/ alanine aminotransferase and glutamic oxalacetic transaminase/aspartate aminotransferase were observed between groups. There was no side effect inflammation and observable pathological damage in the liver tissue of mice after administration. Moreover, collagen deposition and Ishak score were statistically reduced in B.s-CotC-CsSerpin3 spores treated mice. In conclusion, B. subtilis spores displaying CsSerpin3 could be investigated further as an oral vaccine against clonorchiasis.
机译：华支睾吸虫是最严重的食源性寄生虫之一，可导致肝纤维化或胆管癌。目前仍急需有效的华支睾吸虫病预防措施。枯草芽孢杆菌是口服疫苗的有效抗原传递平台。中华Chonorchis sinensis serpin（CsSerpin）被证明是潜在的候选疫苗。在本研究中，CsSerpin3被展示在枯草杆菌孢子表面，重组孢子被口服给BALB/C小鼠。与对照组相比，首次给药后4-8周，粪便、胆汁和肠粘膜中CsSerpin3特异性IgA水平升高。与空白对照组相比，B.s-CotC-CsSerpin3（枯草杆菌孢子的CotC，外壳蛋白）孢子处理组的肠粘膜粘液生成和杯状细胞数量增加。谷氨酸丙酮酸转氨酶/丙氨酸转氨酶和谷氨酸草酰乙酸转氨酶/天冬氨酸转氨酶的活性在两组之间无显著差异。给药后小鼠肝组织无明显的炎症副作用和病理损伤。此外，在B.s-CotC-CsSerpin3孢子处理的小鼠中，胶原沉积和Ishak评分在统计学上降低。总之，显示CsSerpin3的枯草杆菌孢子可以作为一种口服华支睾吸虫病疫苗进行进一步研究。

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