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Broad but restricted detection of malacosporeans in a Neotropical cradle of diversification

机译:在多样化的新颅座中对Malacosehs的广泛但受限制的检测

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摘要

This study undertook the first investigation of malacosporean infections in Neotropical fish. We used polymerase chain reaction detection with a primer set generally targeting known malacosporeans to assay for infection in the kidney of 146 fish in 21 species belonging to 12 families collected from two areas in the Amazon Basin. Infections were found in 13 fish variously belonging to seven species in six families and included the first identification of a malacosporean infection in cartilaginous fish (a freshwater stingray). Based on ssrDNA, all infections represented a single Buddenbrockia species (Buddenbrockia sp. E) that demonstrates an exceptionally broad range of fish species infected, and countered our expectations of high Neotropical malacosporean diversity. Infections were characterized at varying and often high prevalences in fish species but sample sizes were small. Ascertaining whether highly divergent malacosporeans have not been detected by current primers, and more comprehensive sampling may reveal whether malacosporeans are truly as species poor in the Amazon Basin as present data suggest. Our results prompt speculations about evolutionary scenarios including introduction via marine incursions and patterns of host use over time.
机译:这项研究首次对新热带鱼的马拉科斯伯里感染进行了调查。我们使用聚合酶链反应检测法和一套针对已知马来西亚人的引物,对从亚马逊河流域两个地区采集的12科21种146条鱼的肾脏感染进行检测。在6科7种13条鱼中发现了感染,其中包括首次在软骨鱼(一种淡水黄貂鱼)中发现马拉科斯伯雷感染。基于ssrDNA,所有感染都代表了一个单一的布登布鲁克鱼种(Buddenbrockia sp.E),表明感染的鱼类种类非常广泛,这与我们对新热带马达加斯加物种多样性的预期背道而驰。在鱼类中,感染的特点是发病率不同,而且往往很高,但样本量很小。确定目前的引物是否没有检测到高度分化的马拉科斯波人,以及更全面的抽样可能会揭示,马拉科斯波人是否真的像现有数据所表明的那样,在亚马逊河流域物种贫乏。我们的研究结果引发了对进化场景的推测,包括通过海洋入侵引入和宿主随时间的使用模式。

著录项

  • 来源
    《Parasitology》 |2021年第5期|共8页
  • 作者

    Juliana Naldoni;

  • 作者单位

    Departamento de Ciencias Biologicas Universidade Federal de Sao Paulo (UNIFESP) Diadema SP;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 寄生虫病;
  • 关键词

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