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Magnesium supports nitrogen uptake through regulating NRT2.1/2.2 in soybean

机译：镁通过在大豆中调节NRT2.1 / 2.2来支持氮气吸收

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摘要

Aims Nitrogen (N) and magnesium (Mg) are two of the essential elements for plant growth. Application of Mg may increase N use efficiency in plants, but the mechanisms underlying Mg and N interaction are not well understood. The objectives of this study were to assess how Mg supply affects N uptake in soybean, and to further investigate underlying molecular mechanisms. Methods Soybean (Glycine max L.) seedlings were provided with various concentrations of Mg under different N conditions in hydroponic cultures. Biomass, nutrient and sugar concentrations, and gene expression were determined. Tissue specificity of gene expression was performed in hairy-root transformants. Results Increasing Mg supply enhanced N concentration and total N uptake in soybean. The promotive effect is due to the enhanced uptake of nitrate but not ammonium. Moreover, elevating nitrate supply can partially restore growth retardation and leaf chlorosis caused by low Mg availability. A comparative transcriptomic study revealed that two Nitrate Transporter 2 (NRT2) family genes NRT2.1 and NRT2.2 were positively regulated by Mg in roots. Both NRT2.1 and 2.2 are mainly expressed in the exodermis and epidermis of soybean roots, suggesting roles in nitrate uptake. In addition, under low-Mg conditions, expression of NRT2.1 and 2.2 and subsequent N uptake were restored upon exogenous addition of sucrose, implying the possibility that Mg may regulate NRT2.1/2.2 expression through facilitation of sucrose allocation. Conclusions The results of this study indicate that Mg promotes N uptake through positive regulation of NRT2.1/2.2 expression in soybean roots. This Mg-induced gene expression appears to act through-facilitation of sucrose allocation from shoots to roots.
机译：氮和镁是植物生长的两种必需元素。施用镁可以提高植物对氮的利用效率，但镁和氮相互作用的机制尚不清楚。本研究的目的是评估镁供应如何影响大豆对氮的吸收，并进一步研究潜在的分子机制。方法在水培条件下，对大豆幼苗进行不同浓度的镁处理。测定生物量、营养和糖浓度以及基因表达。在毛状根转化体中进行了基因表达的组织特异性。结果增加镁供应可提高大豆的氮浓度和总氮吸收。这种促进作用是由于硝酸盐而非铵的吸收增强。此外，增加硝酸盐供应可以部分恢复低镁有效性导致的生长迟缓和叶片失绿。一项比较转录组学研究显示，两个硝酸盐转运蛋白2（NRT2）家族基因NRT2。1和NRT2。2株在根系中受到Mg的正调控。都是NRT2。1和2.2主要表达于大豆根的外皮层和表皮，表明它们在硝酸盐吸收中起作用。此外，在低镁条件下，NRT2的表达。1和2.2以及随后的氮吸收在外源添加蔗糖后恢复，这意味着镁可能调节NRT2。1/2.2通过促进蔗糖分配表达。结论本研究结果表明，镁通过NRT2的正向调节促进氮的吸收。1/2.2在大豆根中的表达。这种Mg诱导的基因表达似乎是通过促进蔗糖从芽到根的分配而起作用的。

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  • 来源
    《Plant and Soil》 |2020年第2期|共15页
  • 作者

    Peng Wen Ting; Qi Wen Lu; Nie Miao Miao; Xiao Yan Bo; Liao Hong; Chen Zhi Chang;

  • 作者单位

    Fujian Agr &

    Forestry Univ Coll Resources &

    Environm Root Biol Ctr Fuzhou 350002 Fujian Peoples R China;

    Yunnan Minzu Univ Sch Ethnomed &

    Ethnopharm Kunming 650500 Yunnan Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Resources &

    Environm Root Biol Ctr Fuzhou 350002 Fujian Peoples R China;

    Yunnan Minzu Univ Sch Ethnomed &

    Ethnopharm Kunming 650500 Yunnan Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Resources &

    Environm Root Biol Ctr Fuzhou 350002 Fujian Peoples R China;

    Fujian Agr &

    Forestry Univ Coll Resources &

    Environm Root Biol Ctr Fuzhou 350002 Fujian Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 土壤学;
  • 关键词

    Magnesium; Nitrogen uptake; Soybean; NRT2; Sucrose;

    机译：镁;氮吸收;大豆;NRT2;蔗糖;

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