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首页> 外文期刊>Polymers for advanced technologies >Cold atmospheric plasma modification of curcumin loaded in tri-phosphate chitosan nanoparticles enhanced breast cancer cells apoptosis
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Cold atmospheric plasma modification of curcumin loaded in tri-phosphate chitosan nanoparticles enhanced breast cancer cells apoptosis

机译：冷气氛血浆改性姜黄素加载三磷酸壳聚糖纳米粒子增强乳腺癌细胞凋亡

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摘要

The anti-cancer mechanisms of curcumin have been reported to include suppressions of angiogenesis and tumor proliferation. The main goal of this research is to increase the solubility of curcumin by cold atmospheric plasma (CAP) and assess the effects of modified curcumin by charging with tri-polyphosphate chitosan nanoparticles for MCF-7, MDA-MB-231 breast cancer cells, and human fibroblast cells. Curcumin modification was done by CAP and its solubility was evaluated by spectrophotometry. After loading modified curcumin into nano-chitosan-TPP, nanocurcumin was characterized by scanning electron microscopy. Cellular viability and apoptosis of treated cells were assessed by MTT and Annexin V. The changes of messenger RNA expression ofTP5353andVEGFgenes were analyzed by real-time PCR. CAP was able to transform the curcumin to possess hydrophilic characteristics after 90 seconds. The mean diameter of Curcumin loaded chitosannanoparticles (NPs) were determined as 48 nm. MTT results showed that the IC(50)of nano Cur-chitosan-TPP was effectively decreased compared to free curcumin in MCF-7 (15 mu g/mL at 72 hours vs 20 mu g/mL at 48 hours). Additionally, nano Cur-chitosan-TPP had no significant effect on normal cells (Human dermal fibroblas: HDF), whereas it also decreased the viability of triple negative breast cancer cell line (MDA-MB-231). Real-time PCR results showed that expression level ofTP53gene was upregulated (P= .000), whereasVEGFgene downregulated (P= .000) in treated MCF-7 cells. Curcumin loaded chitosan nanoparticles have led to an induction of apoptosis (79.93%) and cell cycle arrest (at S and G2M). Modified-curcumin-tri-polyphosphate chitosan nanoparticles using CAP can be considered as a proper candidate for breast cancer treatment.
机译：姜黄素的抗癌机制包括抑制血管生成和肿瘤增殖。本研究的主要目的是通过冷空气等离子体（CAP）增加姜黄素的溶解度，并通过向MCF-7、MDA-MB-231乳腺癌细胞和人成纤维细胞中加入三聚磷酸壳聚糖纳米粒来评估改性姜黄素的效果。用CAP对姜黄素进行改性，并用分光光度法测定其溶解度。在纳米壳聚糖TPP中负载改性姜黄素后，用扫描电镜对纳米姜黄素进行了表征。MTT法和膜联蛋白V法检测细胞活力和凋亡，实时PCR法检测p5353和vegf基因信使RNA表达的变化。CAP能够在90秒后将姜黄素转化为亲水特性。姜黄素负载的壳聚糖颗粒（NPs）的平均直径为48 nm。MTT结果显示，与MCF-7中的游离姜黄素相比，纳米姜黄素-壳聚糖TPP的IC（50）有效降低（72小时时为15μg/mL，48小时时为20μg/mL）。此外，nano-Cur-壳聚糖TPP对正常细胞（人真皮纤维蛋白：HDF）没有显著影响，但也降低了三阴性乳腺癌细胞系（MDA-MB-231）的活力。实时PCR结果显示，在处理过的MCF-7细胞中，p53基因的表达水平上调（P=0.000），而EGFR基因的表达水平下调（P=0.000）。载姜黄素的壳聚糖纳米粒可诱导细胞凋亡（79.93%）和细胞周期阻滞（S和G2M）。使用CAP修饰的姜黄素-三聚磷酸盐-壳聚糖纳米粒可以被认为是乳腺癌治疗的合适候选者。

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