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首页> 外文期刊>Polymers for advanced technologies >Investigation of cell-free poly lactic acid/nanoclay scaffolds prepared via thermally induced phase separation technique containing hydroxyapatite nanocarriers of erythropoietin for bone tissue engineering applications
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Investigation of cell-free poly lactic acid/nanoclay scaffolds prepared via thermally induced phase separation technique containing hydroxyapatite nanocarriers of erythropoietin for bone tissue engineering applications

机译：通过热诱导的相分离技术研究含有促红细胞素纳米载体的热诱导相分离技术制备的无细胞聚乳酸/纳米粘土支架进行促红细胞生成素骨组织工程应用

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The use of cell-free scaffolds containing bioactive molecules having a controlled released pattern has been proposed as a promising approach in field of tissue engineering. This system would eliminate the challenges associated with cell implantation. For bone tissue engineering, in particular, the environment in favor of both de novo bone formation and vascularization are considered critical approach. Erythropoietin (EPO) has been shown to have bone-related pleiotropic effects. However, due to the adverse systemic side effect, its local administration with controlled release has been recommended. This study aimed to fabricate erythropoietin (EPO)-releasing poly lactic acid (PLA)/nanoclay (NC)/nanohydroxyaptitite (nHA) as a new scaffold for bone tissue engineering application. PLA/NC/nHA-EPO scaffolds were fabricated using thermally induced phase separation technique. The fabricated scaffolds were first characterized in terms of morphology and physical properties, as well as their EPO realizing pattern. Then, their biocompatibility was assessed in response to the MG-63 human osteoblast-like cell line. Finally, its bone regeneration capability was evaluated in a rat calvarial model. The result showed that the fabricated scaffolds presented acceptable physical properties with the sustained release of EPO. The in vitro biocompatibility was also approved. Their bone regeneration in rat calvaria showed that the PLA/NC/nHA-EPO scaffolds were significantly able to generate bone formation (41% after 8 weeks). Also, the formation of new vessels and capillaries were evident. The presence of osteoblast in the defect also confirmed that the EPO was potent in inducing mesenchymal stem cells chemotaxis. The PLA/NC/nHA containing EPO could be a promising cell-free bioscaffold for bone tissue regeneration.
机译：利用含有可控释放模式的生物活性分子的无细胞支架已被认为是组织工程领域一种很有前途的方法。该系统将消除与细胞植入相关的挑战。特别是对于骨组织工程，有利于新生骨形成和血管化的环境被认为是关键的方法。促红细胞生成素（EPO）已被证明具有与骨相关的多效性作用。然而，由于其不良的系统性副作用，建议局部给药并控制释放。本研究旨在制备释放促红细胞生成素（EPO）的聚乳酸（PLA）/纳米粘土（NC）/纳米羟基磷灰石（nHA）作为骨组织工程应用的新支架。采用热诱导相分离技术制备了PLA/NC/nHA-EPO支架。首先从形态学和物理性能以及EPO实现模式方面对所制备的支架进行了表征。然后，评估它们对MG-63人成骨样细胞系的生物相容性。最后，在大鼠颅骨模型中评估其骨再生能力。结果表明，随着EPO的持续释放，所制备的支架呈现出可接受的物理性能。体外生物相容性也得到了认可。它们在大鼠颅骨中的骨再生表明PLA/NC/nHA-EPO支架能够显著生成骨（8周后为41%）。此外，新血管和毛细血管的形成也很明显。成骨细胞在缺损处的存在也证实了EPO在诱导间充质干细胞趋化方面的有效性。含有EPO的PLA/NC/nHA有望成为骨组织再生的无细胞生物支架。

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