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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >An X1 alpha plasmid from a Salmonella enterica serovar Ohio isolate carrying a novel IS26-bounded tet(C) pseudo-compound transposon
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An X1 alpha plasmid from a Salmonella enterica serovar Ohio isolate carrying a novel IS26-bounded tet(C) pseudo-compound transposon

机译：来自沙门氏菌的X1α质粒来自沙门氏菌肠道俄亥俄州分离物，携带新型IS26界TET（C）伪复合转座子

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摘要

The sequence of a conjugative plasmid, pSRC22-2, found in a multiply antibiotic resistant Salmonella enterica serovar Ohio isolate SRC22 originally cultured from swine in 1999, was determined. Plasmid pSRC22-2 has a copy number of approximately 40 and transfers tetracycline resistance at very high frequency. It was typed as IncX1 using the three typing schemes proposed for X-type plasmids, which utilize the replication region, iteron region and taxC conjugation gene and pSRC22-2 belongs to the X1 alpha subgroup. The plasmid backbone, derived by removing mobile elements, is shared with pOLA52, which was the first fully sequenced IncX1 plasmid, and five other X1 alpha plasmids. The pSRC22-2 backbone is interrupted by a complete copy of an IS903 isoform, partial copies of IS1 and IS903 on either side of a 5930 bp IS26-bounded pseudo-compound transposon (PCT), and a novel 256 bp miniature inverted repeat transposable element (MITE). The MITE belongs to the Tn3 family and was named MITESen1. The PCT, which carries a tet(C) tetracycline resistance determinant, is bounded by copies of a novel IS26 variant, IS26-v4, and was designated PTn6184. Comparison of PTn6184 with other tet(C)-carrying PCTs revealed that it can be derived from the largest, PTntet(C), via a two-step process that re-orders the central fragment and involves both an IS26-mediated event and homologous recombination. IS26-v4, which encodes a variant transposase, Tnp26 G184D, has appeared in only 46 entries in the GenBank non-redundant database.
机译：对1999年从猪身上培养的耐多种抗生素的俄亥俄州沙门氏菌血清型分离株SRC22中发现的接合质粒pSRC22-2的序列进行了测定。质粒pSRC22-2的拷贝数约为40，并以非常高的频率转移四环素耐药性。使用针对X型质粒提出的三种分型方案将其分型为IncX1，这三种分型方案利用复制区、iteron区和taxC结合基因，pSRC22-2属于X1α亚群。通过去除移动元件获得的质粒主干与pOLA52共享，pOLA52是第一个完全测序的IncX1质粒，以及其他五个X1α质粒。pSRC22-2主干被IS903亚型的完整拷贝、5930 bp IS26结合伪复合转座（PCT）两侧IS1和IS903的部分拷贝以及一个新的256 bp微型反向重复转座因子（MITE）打断。这种螨属于Tn3家族，被命名为MITESen1。PCT携带一个四环素抗性决定簇，由一个新的IS26变体IS26-v4的拷贝绑定，命名为PTn6184。将PTn6184与其他携带tet（C）的PCT进行比较，发现它可以通过两步过程从最大的PTntet（C）衍生而来，该过程对中心片段进行重新排序，并涉及IS26介导的事件和同源重组。IS26-v4编码一种变异转座酶Tnp26 G184D，它只出现在GenBank非冗余数据库的46个条目中。

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