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首页> 外文期刊>Primates >Effect of bFGF on fibroblasts derived from the golden snub-nosed monkey
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Effect of bFGF on fibroblasts derived from the golden snub-nosed monkey

机译：BFGF对衍生自金黄色鼻猴的成纤维细胞的影响

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摘要

Golden snub-nosed monkeys are endangered animals in China, and their cells have been demonstrated to be important as genetic resources and in applications for advancing biological research. Moreover, in primary research, basic fibroblast growth factor (bFGF) is used to promote the proliferation of fibroblasts to create abundant cells for cryopreservation. To further investigate the effect of bFGF on the efficiency of preservation of fibroblasts obtained from an endangered species, a fibroblast cell line was isolated from a dead golden snub-nosed monkey. Cell viability and mitochondrial membrane potential were assessed using CCK8 and JC-1 assay kits. The karyotype was analyzed by chromosomal microarray analysis, while RNA sequencing and gene expression analyses were performed to assess molecular changes in response to bFGF. Flow cytometry was used to characterize changes in cell surface markers in response to bFGF treatment. The results showed that cells maintained typical fibroblast morphology, while cell viability and mitochondrial membrane potential were not significantly affected between three and eight passages (p > 0.05). We also observed that the addition of bFGF promoted fibroblast proliferation and increased mitochondrial membrane potential. In addition, the bFGF treatment did not alter the normal karyotype of cells, downregulating fibroblast-associated genes and upregulating those associated with cell regulation, including those of the WNT, PI3K and MAPK pathways. The addition of bFGF also increased CD29, CD90, CD105, CD34 and CD44 expression while decreasing that of CD14 and HLA-DR at the protein level. Taken together, these results demonstrate that bFGF may upregulate the WNT, PI3K and MAPK pathways to promote cell proliferation while also increasing the expression of genes and surface markers associated with mesenchymal and hematopoietic cell linages.
机译：金丝猴是中国的濒危动物，它们的细胞已被证明是重要的遗传资源，并在推进生物学研究的应用中发挥着重要作用。此外，在初步研究中，碱性成纤维细胞生长因子（bFGF）被用于促进成纤维细胞的增殖，以创造大量用于冷冻保存的细胞。为了进一步研究bFGF对濒危物种成纤维细胞保存效率的影响，从一只死亡的金丝猴中分离出一株成纤维细胞系。使用CCK8和JC-1检测试剂盒评估细胞活力和线粒体膜电位。通过染色体微阵列分析核型，同时进行RNA测序和基因表达分析，以评估对碱性成纤维细胞生长因子反应的分子变化。流式细胞术用于表征细胞表面标记物在碱性成纤维细胞生长因子处理后的变化。结果表明，细胞保持典型的成纤维细胞形态，而细胞活力和线粒体膜电位在第三代和第八代之间没有显著影响（p>0.05）。我们还观察到，添加bFGF促进成纤维细胞增殖，增加线粒体膜电位。此外，bFGF治疗没有改变细胞的正常核型，下调成纤维细胞相关基因，上调与细胞调节相关的基因，包括WNT、PI3K和MAPK途径的基因。bFGF的加入也增加了CD29、CD90、CD105、CD34和CD44的表达，同时在蛋白质水平上降低了CD14和HLA-DR的表达。总之，这些结果表明，bFGF可能上调WNT、PI3K和MAPK通路，促进细胞增殖，同时增加与间充质细胞和造血细胞系相关的基因和表面标记物的表达。

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  • 来源
    《Primates》 |2021年第2期|共10页
  • 作者

    Liu Xin; Wei Hao; Yang Jing; Li Jingjing; Zhang Pei; Li Yuli; Hou Rong; Hua Jinlian; Yan Xingrong; Li Baoguo;

  • 作者单位

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Xi An Jiao Tong Univ Sch Life Sci &

    Technol Xian 710049 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Agr &

    Forestry Univ Coll Vet Med Yangling 712100 Shaanxi Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

    Northwest Univ Key Lab Resource Biol &

    Biotechnol Western China Shaanxi Key Lab Anim Conservat Minist Educ Coll Life Sci Xian 710069 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 动物分类学（系统动物学）;
  • 关键词

    Golden snub-nosed monkey; Fibroblast; Gene expression; bFGF; RNA sequencing;

    机译：Golden Snub-nosed猴子;成纤维细胞;基因表达;BFGF;RNA测序;

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