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首页> 外文期刊>Primates >Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil
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Genetic analysis of an insular population of Sapajus nigritus (Primates: Cebidae) in Rio de Janeiro state, Brazil

机译:巴西里约热内卢州萨巴斯尼克尼斯(灵长类动物:Cebidae)遗传分析

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摘要

The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.
机译:黑角卷尾猴(Sapajus nigritus)是一种新热带灵长类动物,广泛分布于巴西东南部至阿根廷东北部。尽管该物种被描述为具有被毛模式变异,甚至具有种群内差异,并且在沙巴胡斯物种中具有最大的遗传多样性,但对自然种群的研究仍然很少有助于了解这种种内变异。我们研究了巴西里约热内卢州伊尔哈·达·马兰巴亚(Ilha da Marambaia,Rio de Janeiro,RJ)尚未研究的人群中的个体,并将其与已发表的黑链球菌数据进行了比较。我们试图通过C-显带和荧光原位杂交,用#11qHe+/21WCP探针进行染色体组成性异染色质(He+)模式,并用细胞色素C氧化酶I和II基因序列进行系统发育分析,通过表型和遗传特征来确认该物种。被毛呈现出两种颜色模式,从身体上的棕色到黑色,胸部的黄色到棕色,面部的白色到黄色,除了头部的簇毛的存在和形状,对应于黑色链球菌。He+在第4、12、13和17对中进行了鉴定,而在第6、19和21对中则不太一致,这已经为该物种进行了描述。虽然大多数Sapajus物种都有一个大的He+块,但在这里,第11对被鉴定为不含中心外He+,这与阿根廷报道的S.nigritus相同。分子分析显示,该群体与其他黑链球菌序列存在差异,这加强了RJ样本与分布的其他部分进行比较时已经证明的趋势,这可能代表了进化偏差。

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