The basement membrane protein nidogen-1 supports platelet adhesion and activation

Lakshmanan Hari Hara Sudhan; Melrose Alexander R.; Sepp Anna-Liisa I; Mitrugno Annachiara; Ngo Anh T. P.; Khader Ayesha; Thompson Rachel; Sallee Daniel; Pang Jiaqing; Mangin Pierre H.; Jandrot-Perrus Martine; Aslan Joseph E.; McCarty Owen J. T.

首页> 外文期刊>Platelets >The basement membrane protein nidogen-1 supports platelet adhesion and activation

【24h】

The basement membrane protein nidogen-1 supports platelet adhesion and activation

机译：基底膜蛋白Nidogen-1支持血小板粘附和活化

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The core structure of the extracellular basement membrane is made up of self-assembling networks of collagen and laminin which associate with each other through the bridging adapter proteins including the sulfated monomeric glycoprotein nidogen. While collagen and laminin are known to support platelet adhesion and activation via beta 1 integrins and glycoprotein (GP) VI, respectively, whether nidogen contributes to platelet activation and hemostasis is unknown. In this study, we demonstrate that recombinant human nidogen-1 supports platelet adhesion and stimulates platelet activation in a phospholipase-C gamma-2 (PLC gamma 2), Src and Syk kinase-dependent manner downstream. Platetet adhesion to nidogen-1 was inhibited by blocking the platelet receptors GPVI and beta 1 integrins. Platelet adhesion to nidogen-1 activated the I kappa B kinase (IKK) complex, while pharmacological inhibition of IKK blocked platelet spreading on nidogen. Taken together our results suggest that nidogen may play a redundant role in hemostasis by activating platelets downstream of GPVI.

机译：细胞外基膜的核心结构由胶原和层粘连蛋白的自组装网络组成，它们通过桥接适配器蛋白质相互结合，包括硫酸化单体糖蛋白巢蛋白。虽然已知胶原蛋白和层粘连蛋白分别通过β1整合素和糖蛋白（GP）VI支持血小板粘附和活化，但巢蛋白是否有助于血小板活化和止血尚不清楚。在这项研究中，我们证明重组人巢蛋白-1支持血小板粘附，并以磷脂酶-C-γ-2（PLC-γ2）、Src和Syk激酶依赖的方式在下游刺激血小板活化。通过阻断血小板受体GPVI和β1整合素，血小板与巢蛋白-1的粘附受到抑制。血小板与巢原-1的粘附激活了I-κB激酶（IKK）复合物，而对IKK的药理学抑制阻止了血小板在巢原上的扩散。综上所述，我们的研究结果表明，巢原可能通过激活GPVI下游的血小板在止血中发挥多余的作用。

著录项

来源
《Platelets》 |2021年第3期|共5页
作者
Lakshmanan Hari Hara Sudhan; Melrose Alexander R.; Sepp Anna-Liisa I; Mitrugno Annachiara; Ngo Anh T. P.; Khader Ayesha; Thompson Rachel; Sallee Daniel; Pang Jiaqing; Mangin Pierre H.; Jandrot-Perrus Martine; Aslan Joseph E.; McCarty Owen J. T.;
展开▼
作者单位

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Knight Cardiovasc Inst Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Univ Strasbourg FMTS BPPS UMR S 1255 EFS Grand Est INSERM Strasbourg France;

Univ Paris Diderot INSERM Sorbonne Paris Cite Lab Vasc Translat Sci Paris France;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

Oregon Hlth &

Sci Univ Dept Biomed Engn 3303 SW Bond Ave CH13B Portland OR 97239 USA;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
Extracellular matrix proteins; hemostasis; nidogen; platelet;

机译：细胞外基质蛋白质;止血;氮素;血小板;

相似文献

外文文献
中文文献
专利

1. Activated changes of platelet ultra microstructure and plasma granule membrane protein 140 in patients with non-small cell lung cancer [J] . ZHUGE Yi, ZHOU Jian-ying, YANG Guang-die, 中华医学杂志（英文版） . 2009,第009期
2. Characterization of Platelet Activating Factor(PAF)Binding Sites in Rabbit Platelet Membranes [J] . 无中国药学（英文版） . 1993,第001期
3. Platelet-activating factor mediates hydrogen peroxide induced endothelial-leukocyte adhesion [J] . 丁自强, 李少华, 吴中立中国人民解放军军医大学学报：英文版 . 1992,第003期
4. Investigation of basement membrane proteins in a case of granular cell ameloblastoma [J] . Puangwan Lapthanasupkul, Sopee Poomsawat, Jira Chindasombatjaroen 国际口腔科学杂志（英文版） . 2012,第001期
5. Anti-angiogenic effect of the basement membrane protein nidogen-1 in a mouse model of choroidal neovascularization (vol 118, pg 80, 2014) [J] . Semkova Irina, Kociok Norbert, Karagiannis Dimitrios, Experimental Eye Research . 2015,第Null期

机译：基底膜蛋白nidogen-1在脉络膜新生血管小鼠模型中的抗血管生成作用（第118卷，第80页，2014年）
6. Basement membrane protein nidogen-1 is a target of meprin beta in cisplatin nephrotoxicity [J] . Herzog Christian, Marisiddaiah Raju, Haun Randy S., Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research . 2015,第2期

机译：基底膜蛋白nidogen-1是甲氨蝶呤β顺铂肾毒性的靶标
7. Anti-angiogenic effect of the basement membrane protein nidogen-1 in a mouse model of choroidal neovascularization (vol 118, pg 80, 2014) [J] . Semkova Irina, Kociok Norbert, Karagiannis Dimitrios, Experimental Eye Research . 2015,第Null期

机译：脉络膜新生血管小鼠模型中基底膜蛋白氮素-1的抗血管生成效果（Vol 118，PG 80,2014）
8. Protein Adsorption and Affects on Platelet Adhesion and Bacterial Adhesion on Submicron Textured Biomaterial Surfaces [C] . Li-Chong Xu, Christopher A. Siedlecki Society for Biomaterials annual meeting and exposition . 2014

机译：蛋白吸附及其对亚微米质感生物材料表面上血小板粘附和细菌粘附的影响
9. Evaluating surface induced platelet adhesion and activation with surface patterning and protein immobilization techniques. [D] . Corum, Lindsey Elizabeth. 2011

机译：使用表面图案和蛋白质固定技术评估表面诱导的血小板粘附和激活。
10. Basement membrane protein nidogen-1 is a target of meprin β in cisplatin nephrotoxicity [O] . Christian Herzog, Raju Marisiddaiah, Randy S. Haun, -1

机译：基底膜蛋白nidogen-1是甲氨蝶呤β顺铂肾毒性的靶标
11. Basement membrane protein nidogen-1 is a target of meprin β in cisplatin nephrotoxicity [O] . Christian Herzog, Raju Marisiddaiah, Randy S. Haun, 2015

机译：基底膜蛋白氮素-1是顺铂肾毒性Meprinβ的靶标
12. Identification of Proteins Interacting at Blood or Plasma/Biomaterial Interfaces: Relation to Platelet and White Cell Adhesion, and to Clotting, and to Interactions Among White Cells and Platelets at Interfaces [R] . Vroman, L. , Munoz, P. C. 1981

机译：在血液或血浆/生物材料界面相互作用的蛋白质的鉴定：与血小板和白细胞粘附，凝血以及界面处白细胞和血小板之间的相互作用的关系

The basement membrane protein nidogen-1 supports platelet adhesion and activation

摘要

著录项

相似文献

相关主题

期刊订阅