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首页> 外文期刊>Platelets >Effects of pH and concentration of sodium citrate anticoagulant on platelet aggregation measured by light transmission aggregometry induced by adenosine diphosphate
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Effects of pH and concentration of sodium citrate anticoagulant on platelet aggregation measured by light transmission aggregometry induced by adenosine diphosphate

机译：用腺苷二磷酸盐诱导的光透射聚集体测量柠檬酸钠抗凝剂钠对血小板聚集的影响

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The 2013 ISTH-SSC guidelines for the standardization of light transmission aggregometry (LTA) were largely based on expert consensus, as studies directly comparing LTA methodologies were lacking. We experimentally tested the cogency of ISTH-SSC recommendations pertaining to use of anticoagulant, in particular whether: (1) buffered citrate (BC) is preferable to unbuffered citrate (C); (2) the two recommended concentrations of sodium citrate (109 and 129 mM) are equivalent in terms of platelet aggregation (PA). Blood from 16 healthy volunteers was collected into BC and C (109 and 129 mM). PA was measured by LTA in platelet-rich plasma (PRP) stimulated by adenosine diphosphate (ADP) (2 M) immediately after PRP preparation and up to 4 hr after blood collection; pH and platelet counts in PRP were measured in parallel. pH in PRP increased with time up to about 8 for all anticoagulants, although it was lower in BC than in C at all times. In BC, PA was lower at 45 min, but equivalent at all other times. PA was higher and more stable in sodium citrate 109 mM than in 129 mM at all times. The extent of PA did not change for up to 2 hr after blood collection, and subsequently dramatically decreased. In contrast with ISTH-SSC recommendations, (1) BC does not show advantages compared to C; (2) 109 mM citrate is preferable to 129 mM, because it better supports PA; and (3) LTA studies should be completed within 2 hr of blood collection, instead of the recommended 4 hr.
机译：2013年ISTH-SSC关于光透射聚集仪（LTA）标准化的指南主要基于专家共识，因为缺乏直接比较LTA方法的研究。我们通过实验测试了ISTH-SSC关于抗凝剂使用的建议的有效性，尤其是：（1）缓冲柠檬酸盐（BC）是否优于非缓冲柠檬酸盐（C）；（2） 两种推荐浓度的柠檬酸钠（109和129 mM）在血小板聚集（PA）方面相当。从16名健康志愿者的血液中采集到BC和C（109和129毫米）。在PRP制备后立即和采血后4小时内，通过LTA在二磷酸腺苷（ADP）（2 M）刺激的富血小板血浆（PRP）中测量PA；同时测量PRP中的pH值和血小板计数。对于所有抗凝剂，PRP中的pH值随着时间增加而增加，直到8左右，尽管BC中的pH值始终低于C中的pH值。在BC，PA在45分钟时较低，但在所有其他时间都相当。在任何时候，109mm柠檬酸钠溶液中的PA都比129mm柠檬酸钠溶液中的PA更高、更稳定。在采血后2小时内，PA的范围没有变化，随后显著降低。与ISTH-SSC建议相比，（1）BC与C相比没有优势；（2） 109mm柠檬酸盐比129mm柠檬酸盐更好，因为它更好地支持PA；（3）LTA研究应在采血后2小时内完成，而不是建议的4小时。

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