• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Platelets >How to perform aggregometry and lumi-aggregometry in mouse platelets
【24h】

How to perform aggregometry and lumi-aggregometry in mouse platelets

机译：如何在鼠标血小板中执行聚合测量和lumi-aggremetry

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Light transmission aggregometry and lumi-aggregometry are the gold standard platelet assays both clinically and for basic research. The availability of different strains of genetically modified mice and mouse models of human disease means that often laboratories need to use mouse platelets in these assays. Overall, performing aggregometry and lumi-aggregometry with mouse platelets is similar to with human platelets, although methods need be adapted to accommodate their small size, reduced blood volume, and different protein levels. This review aims to highlight these key considerations when planning aggregometry experiments with mouse platelets. These include the method of taking blood, including the use of anticoagulants, as well as the method of platelet preparation, and how to maximize yields. This review also covers how to maximize the number of aggregations that can be performed, both by understanding the minimum requirements of your aggregometer, or by considering new approaches. These include employing high throughput plate-based aggregometry (Optimul), or the use of TPO-mimetics to stimulate platelet production in mice to boost their platelet counts. Finally, phenotypic differences between mouse and human platelets, such as protein expression or sensitivity to agonists are discussed as an important consideration when planning experiments.
机译：光透射法和lumi法是临床和基础研究中血小板检测的金标准。不同品系的转基因小鼠和人类疾病小鼠模型的可用性意味着实验室通常需要在这些分析中使用小鼠血小板。总的来说，对小鼠血小板进行聚集测定和lumi聚集测定与人类血小板相似，尽管需要调整方法以适应其体积小、血容量减少和不同的蛋白质水平。本综述旨在强调在规划小鼠血小板聚集计量试验时的这些关键考虑因素。其中包括采血方法，包括抗凝剂的使用，以及血小板制备方法，以及如何最大限度地提高产量。本综述还介绍了如何通过了解聚合计的最低要求或考虑新方法，最大限度地增加可执行的聚合数量。其中包括采用高通量平板聚集测定法（Optimul），或使用TPO模拟物刺激小鼠血小板生成，以提高其血小板计数。最后，小鼠和人类血小板之间的表型差异，例如蛋白质表达或对激动剂的敏感性，将作为计划实验时的一个重要考虑因素进行讨论。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号