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Effects of platelet-rich plasma on liver regeneration in CCl4-induced hepatotoxicity model

机译：富含血小板血浆对CCL4诱导的肝毒性模型肝再生的影响

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Numerous bioactive growth factors and cytokines in platelet-rich plasma (PRP) have recently made it an attractive biomaterial for therapeutic purposes. These growth factors have the potential to regenerate the injured tissues. The aim of this study was to investigate the therapeutic effects of PRP in hepatotoxic animal model. Hepatotoxicity was induced in rats by oral administration of 4 mL/kg/week of CCl4 diluted 1:1 in corn oil for 10 weeks. To confirm the hepatotoxicity, 24 h after the last CCl4 administration, blood samples were collected via cardiac puncture to assess the serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, albumin, total protein, and total bilirubin. Twenty-four hours after blood collection, the experimental animals received a single injection of PRP (1 mL) via the anterior mesenteric vein. One week later, all biochemical tests were performed again, and the rats were scarified and their livers were removed, prepared histologically, and stained. The stereological analyses were performed to evaluate the effects of PRP on histopathological features of CCl4-treated livers. The results were compared statistically with the corresponding control and CCl4+normal saline (NS)-treated animals. A significant decrease in the number and volume of hepatocytes (p = 0.01), and also a reduction in the volume of sinusoids (p = 0.001) and connective tissue (p = 0.04), were observed in the PRP-treated animals compared with the CCl4+NS-treated ones. Our findings demonstrated that application of PRP had beneficial effects on CCl4-induced fibrosis; however, it had detrimental effects on the total number of hepatocytes and the volume of hepatocytes and sinusoidal spaces.
机译：富含血小板血浆（PRP）中的多种生物活性生长因子和细胞因子使其成为一种极具吸引力的生物治疗材料。这些生长因子具有再生受损组织的潜力。本研究旨在探讨PRP对肝毒性动物模型的治疗作用。在大鼠中，通过口服4毫升/千克/周的CCl4（在玉米油中以1:1稀释）10周来诱导肝毒性。为了确认肝毒性，在最后一次CCl4给药24小时后，通过心脏穿刺采集血样，以评估血清丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶、白蛋白、总蛋白和总胆红素水平。采血24小时后，实验动物通过肠系膜前静脉单次注射PRP（1毫升）。一周后，再次进行所有生化测试，将大鼠翻松，取出肝脏，进行组织学准备和染色。进行体视学分析，以评估PRP对CCl4处理的肝脏组织病理学特征的影响。将结果与相应的对照组和CCl4+生理盐水（NS）处理的动物进行统计学比较。与CCl4+NS处理的动物相比，PRP处理的动物肝细胞数量和体积显著减少（p=0.01），窦状体（p=0.001）和结缔组织体积显著减少（p=0.04）。我们的研究结果表明，应用PRP对CCl4诱导的纤维化有有益的作用；然而，它对肝细胞总数、肝细胞体积和窦腔有不利影响。

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