• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Platelets >Histological comparison of Platelet rich fibrin clots prepared by fixed-angle versus horizontal centrifugation
【24h】

Histological comparison of Platelet rich fibrin clots prepared by fixed-angle versus horizontal centrifugation

机译：固定角度与水平离心制备的血小板富纤维蛋白凝块的组织学比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Platelet-rich fibrin (PRF) is prepared from whole blood without any exogenous coagulation factors. Several preparation methods have now been introduced, particularly with differences in centrifugation parameters including g-force and time to improve their regenerative potential. Nevertheless, the centrifugation systems have not yet been clearly investigated for their influences on the PRF clot properties. The aim of the present study was to visually and histologically characterize the cell separation manner and blood cell localization on the whole PRF clots prepared by two different centrifugation system, fixed-angle and horizontal centrifugation. Leukocyte- and platelet-rich fibrin (L-PRF) was prepared on a fixed-angle centrifuge machine (IntraSpin, Intra-Lock, FL, USA) at 2700 rpm (similar to 400 g at the RCF-clot; similar to 700 g at the RCF-max) for 12 min. The PRF prepared by horizontal centrifugation was prepared on a horizontal centrifugation (H-PRF) (Eppendorf 5702, Eppendorf, Germany) at 700 g at the RCF-max for 8 min. The cell morphology and localization were observed on the surface of PRF clots by scanning electron microscopy (SEM) and histologically by transaxial frozen sections by means of a film method. L-PRF clots demonstrated a sloped separation between the upper plasma and the bottom red blood cell (RBC) layers according to the angle of the rotor. Red dots were often observed on the distal walls of the tubes in the upper layers, consisting of aggregations of RBCs, leukocytes and platelets by SEM and histology. Clots produced on the horizontal centrifuge showed much smoother cell layer distribution/separation along the tube surfaces when compared to L-PRF. Horizontal centrifugation also demonstrated more evenly distributed platelets throughout the PRF clots when compared to L-PRF that gathered the majority of cells along the distal tube surface or within the buffy-coat region. In summary, it was found that horizontal centrifugation resulted in a more uniform blood cell separation of PRF clots when compared to the accumulation of cells gathered along the distal tube surfaces produced prepared by fixed-angle centrifugation. Future research is needed to evaluate the benefit of horizontal centrifugation in clinical practice.
机译：富含血小板的纤维蛋白（PRF）由全血制备，不含任何外源性凝血因子。现在已经介绍了几种制备方法，尤其是离心参数的差异，包括g-力和时间，以提高它们的再生潜力。然而，离心系统对PRF凝块性质的影响尚未得到明确的研究。本研究的目的是通过两种不同的离心系统，即固定角度离心和水平离心，对整个PRF凝块的细胞分离方式和血细胞定位进行视觉和组织学表征。富含白细胞和血小板的纤维蛋白（L-PRF）在固定角度离心机（美国佛罗里达州IntraSpin，Intra Lock）上以2700 rpm（类似于RCF凝块的400 g；类似于RCF最大值的700 g）制备12 min。通过水平离心制备的PRF在RCF最大值为700 g的水平离心（H-PRF）（德国Eppendorf 5702）上制备8 min通过扫描电子显微镜（SEM）观察PRF凝块表面的形态和定位，并通过薄膜法经轴冷冻切片进行组织学观察。根据转子的角度，L-PRF凝块显示上部血浆和底部红细胞（RBC）层之间的倾斜分离。通过扫描电镜和组织学观察，在上层管的远侧壁上经常观察到红点，由红细胞、白细胞和血小板聚集而成。与L-PRF相比，卧式离心机产生的凝块在试管表面的细胞层分布/分离更加平滑。水平离心法也显示，与L-PRF相比，血小板在整个PRF凝块中的分布更均匀，L-PRF聚集了大部分细胞沿远端管表面或在棕黄色外套区域内。总之，我们发现，与通过固定角度离心制备的沿远端管表面聚集的细胞相比，水平离心使PRF凝块的血细胞分离更加均匀。未来的研究需要评估水平离心在临床实践中的益处。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号