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首页> 外文期刊>The European Journal of Neuroscience >Long-term neural regeneration following injury to the peroneal branch of the sciatic nerve in sheep
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Long-term neural regeneration following injury to the peroneal branch of the sciatic nerve in sheep

机译：在绵羊中坐骨神经伤害后的长期神经再生

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摘要

Peripheral nerves (PNs) are frequently injured as a result of trauma or disease. Development of therapies to regenerate PNs requires the use of animal models, typically beginning in rodents and progressing to larger species. There are several large animal models of PN regeneration that each has their benefits and drawbacks. Sheep have been used in PN studies due to their similarities in body weight to humans and the ease and lesser expense in their care and housing relative to other species. We have investigated the use of sheep for studies of PN regeneration and have developed and tested an injury model in the peroneal branch of the sciatic nerve. Three experimental groups were tested on mature sheep: a bisection; a 5-cm reverse autograft; and sham surgery. Protocols were developed for the post-operative care for animals with this injury, and regeneration was tracked for extended time points via compound muscle action potentials (CMAPs) and endpoint assessments of nerve morphometry, muscle mass and muscle fibrosis. Results indicate the practical viability of this PN injury model and show distinctions in the degree and rate of regeneration between bisection and reverse autograft that persisted 14 months. This long-term study shows bisections lead to significantly improved CMAPS and muscle mass and lesser muscle fibrosis as compared to reverse autograft. The persistence of these discernable changes between two relatively similar experimental groups out to extended time points is an indication of the sensitivity of this nerve section and its potential applicability for comparative studies.
机译：周围神经（PNs）经常因创伤或疾病而受伤。开发再生PNs的疗法需要使用动物模型，通常从啮齿类动物开始，然后发展到更大的物种。PN再生有几种大型动物模型，每种模型都有各自的优点和缺点。绵羊被用于PN研究，因为它们的体重与人类相似，而且与其他物种相比，它们在护理和住宿方面更容易，花费更少。我们研究了绵羊在PN再生研究中的应用，并开发和测试了坐骨神经腓支损伤模型。在成熟绵羊身上测试了三个实验组：二等分；5厘米反向自体移植；还有假手术。为患有这种损伤的动物制定了术后护理方案，并通过复合肌肉动作电位（CMAP）和神经形态计量学、肌肉质量和肌肉纤维化的终点评估，跟踪延长时间点的再生。结果表明了这种PN损伤模型的实际可行性，并且显示了在持续14个月的二等分和反向自体移植之间再生的程度和速率的差异。这项长期研究表明，与反向自体移植相比，二分法可显著改善CMAP和肌肉质量，减少肌肉纤维化。在两个相对相似的实验组之间，这些可识别的变化持续到延长的时间点，这表明了该神经段的敏感性及其在比较研究中的潜在适用性。

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