...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The international journal of developmental biology >Evaluation of morphokinetic characteristics of zona pellucida free mouse pre-implantation embryos using time-lapse monitoring system
【24h】

Evaluation of morphokinetic characteristics of zona pellucida free mouse pre-implantation embryos using time-lapse monitoring system

机译：使用时间流逝监测系统评估Zona Pellucida自由小鼠预植入胚胎的流通特性

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mammalian zygote cleaves and develops to blastocyst within the zona pellucida (ZP) in vivo. The presence or absence of ZP may affect the characteristics of the embryo, including blastomere alignment, cell-cell junction, and compaction.This study aimed to comparethe morphokinetic characteristics of ZP-intact and ZP-free mouse pre-implantation embryos with a time-lapse monitoring system. Mouse 2-cell embryos were collected 1.5 days post coitum (dpc), and their ZPs were removed by treatment with acidTyrode's solution. All embryos were cultured in vitro up to the outgrowth stage at 7.5 dpc. In this study, ZP did not influence the cumulative times from 2-cell to further stages and blastulation. Interestingly, ZP-free embryos at 4-cell stage have three patterns of blastomere alignment according to the number of contact points between blastomeres. However, blastomere alignment did not lead to any differences in morphokinetic comparisons. Regardless of the presence or absence of ZP, embryos compacted after the 8-cell stage took shorter time to become blastocysts than embryos compacted pre-8-cell stage. Nevertheless, cell-cell junction proteins required for successful compaction were similarly expressed between ZP-intact and ZP-free embryos. ZP-intact embryos compacted post-8-cell stage had a higher rate of reaching blastocysts than compacted ZP-intact embryos before 8-cell stage, while the outgrowth/blastocyst rate was similar. In this study, the presence or absence of ZP did not influence embryonic development and expression of cell surface glycoproteins, whereas compaction timing may be one of the criteria for evaluating embryo quality. ZP-free embryos may become an alternative for overcoming cases with ZP problems in human ART programs.
机译：哺乳动物受精卵在体内透明带（ZP）内分裂并发育成胚泡。ZP的存在或缺失可能会影响胚胎的特征，包括卵裂球排列、细胞间连接和紧密性。本研究旨在通过延时监测系统比较ZP完整和无ZP小鼠植入前胚胎的形态动力学特征。性交后1.5天（dpc）收集小鼠2细胞胚胎，并用acidTyrode溶液处理去除其ZP。所有胚胎均在体外培养至7.5 dpc的生长期。在本研究中，ZP不影响从2-细胞到进一步阶段和囊胚的累积时间。有趣的是，根据卵裂球之间接触点的数量，4细胞期的无ZP胚胎有三种卵裂球排列模式。然而，卵裂球排列并没有导致形态动力学比较的任何差异。无论是否存在ZP，8细胞期后致密化的胚胎形成囊胚的时间比8细胞期前致密化的胚胎短。然而，在ZP完整和无ZP的胚胎中，成功压实所需的细胞-细胞连接蛋白表达相似。与8细胞期之前的ZP完整胚胎相比，8细胞期之后的ZP完整胚胎达到囊胚的比率更高，而生长/囊胚的比率相似。在这项研究中，ZP的存在与否并不影响胚胎发育和细胞表面糖蛋白的表达，而压实时间可能是评估胚胎质量的标准之一。无ZP的胚胎可能成为人类ART项目中克服ZP问题的替代方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号