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首页> 外文期刊>The international journal of developmental biology >Measuring functional brain recovery in regenerating planarians by assessing the behavioral response to the cholinergic compound cytisine
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Measuring functional brain recovery in regenerating planarians by assessing the behavioral response to the cholinergic compound cytisine

机译：通过评估对胆碱能化合物细胞素的行为应对来测量再生平面图中的功能性大脑回收

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Planarians are traditional model invertebrates in regeneration and developmental biology research that also display a variety of quantifiable behaviors useful to screen for pharmacologically active compounds. One such behavior is the expression of seizure-like movements (pSLMs) induced by a variety of substances. Previous work from our laboratory showed that cocaine, but not nicotine, induced pSLMs in intact but not decapitated planarians. Interestingly, as decapitated planarians regenerated their heads, they gradually recovered their sensitivity to cocaine. These results suggested a method to assess planarian brain regeneration and a possible way of identifying compounds that could enhance or hold back brain regeneration. In the present work, we demonstrate that the cholinergic agent cytisine is a suitable reference compound to apply our method. Cytisine induces pSLMs in a concentration-dependent manner in intact (but not decapitated) planarians of the species Girardia tigrina. Based on our data, we developed a behavioral protocol to assess planarian brain regeneration over time. We tested this method to measure the effect of ethanol on G. tigrina's brain regeneration. We found that ethanol slows down the rate of planarian brain regeneration in a concentration-dependent manner, consistently with data from other research groups that tested ethanol effects on planarian brain regeneration using different behavioral protocols. Thus, here we establish a general method using cytisine-induced pSLMs as an indicator of brain regeneration in planarians, a method that shows potential for assessing the effect of pharmacologically active compounds in this process.
机译：涡虫是再生和发育生物学研究中的传统模式无脊椎动物，也表现出各种可量化的行为，有助于筛选药理活性化合物。其中一种行为是由多种物质诱发的癫痫样运动（pSLMs）的表达。我们实验室之前的研究表明，可卡因（而非尼古丁）在完整但未斩首的涡虫体内诱发PSLM。有趣的是，随着被斩首的涡虫再生头部，他们逐渐恢复了对可卡因的敏感性。这些结果提出了一种评估扁桃体脑再生的方法，以及一种可能的方法来识别能够增强或抑制脑再生的化合物。在目前的工作中，我们证明胆碱能剂cytisine是应用我们的方法的合适参考化合物。Cytisine以浓度依赖的方式在完整（但不是断头）的虎斑瓢虫中诱导pSLMs。根据我们的数据，我们制定了一个行为方案来评估扁桃体脑随时间的再生。我们测试了这种方法，以测量乙醇对虎纹夜蛾大脑再生的影响。我们发现，乙醇以浓度依赖的方式减缓扁桃体脑再生的速度，这与其他研究小组使用不同的行为方案测试乙醇对扁桃体脑再生的影响的数据一致。因此，我们在这里建立了一种通用方法，使用胞苷诱导的pSLMs作为涡虫脑再生的指标，该方法显示了评估该过程中药理活性化合物效果的潜力。

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