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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Surface pH changes suggest a role for H+/OH- channels in salinity response of Chara australis
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Surface pH changes suggest a role for H+/OH- channels in salinity response of Chara australis

机译：表面pH变化表明Chara Australis的盐度响应中的H + / OH-通道的作用

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To understand salt stress, the full impact of salinity on plant cell physiology has to be resolved. Electrical measurements suggest that salinity inhibits the proton pump and opens putative H+/OH- channels all over the cell surface of salt sensitive Chara australis (Beilby and Al Khazaaly 2009; Al Khazaaly and Beilby 2012). The channels open transiently at first, causing a characteristic noise in membrane potential difference (PD), and after longer exposure remain open with a typical current-voltage (I/V) profile, both abolished by the addition of 1 mM ZnCl2, the main known blocker of animal H+ channels. The cells were imaged with confocal microscopy, using fluorescein isothiocyanate (FITC) coupled to dextran 70 to illuminate the pH changes outside the cell wall in artificial fresh water (AFW) and in saline medium. In the early saline exposure, we observed alkaline patches (bright fluorescent spots) appearing transiently in random spatial distribution. After longer exposure, some of the spots became fixed in space. Saline also abolished or diminished the pH banding pattern observed in the untreated control cells. ZnCl2 suppressed the alkaline spot formation in saline and the pH banding pattern in AFW. The osmotic component of the saline stress did not produce transient bright spots or affect banding. The displacement of H+ from the cell wall charges, the H+/OH- channel conductance/density, and self-organization are discussed. No homologies to animal H+ channels were found. Salinity activation of the H+/OH- channels might contribute to saline response in roots of land plants and leaves of aquatic angiosperms.
机译：为了理解盐胁迫，必须解决盐分对植物细胞生理的全面影响。电学测量结果表明，盐度抑制质子泵，并在盐敏感的澳大利亚轮藻细胞表面打开假定的H+/OH-通道（Beilby and Al-Khazaaly 2009；Al-Khazaaly and Beilby 2012）。通道最初短暂打开，导致膜电位差（PD）中出现特征性噪声，长时间暴露后，以典型的电流-电压（I/V）曲线保持打开状态，这两种情况都被添加1 mM ZnCl2消除，ZnCl2是动物H+通道的主要已知阻断剂。用共焦显微镜对细胞成像，使用异硫氰酸荧光素（FITC）与葡聚糖70偶联，以照亮人工淡水（AFW）和盐水介质中细胞壁外的pH变化。在盐水暴露的早期，我们观察到碱性斑（明亮的荧光点）以随机的空间分布短暂出现。在长时间曝光后，一些斑点在太空中变得固定。生理盐水也消除或减少了未经处理的对照细胞中观察到的pH显带模式。ZnCl2抑制了盐水中碱性斑点的形成和AFW中的pH带模式。盐胁迫的渗透成分不会产生短暂的亮点或影响条带。讨论了H+从细胞壁电荷中的位移、H+/OH-通道电导/密度和自组织。未发现与动物H+通道的同源性。盐分激活H+/OH-通道可能有助于陆地植物根系和水生被子植物叶片的盐分反应。

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