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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Transcriptome profiling provides insights into the fruit color development of wildLycium ruthenicumMurr. from Qinghai-Tibet Plateau
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Transcriptome profiling provides insights into the fruit color development of wildLycium ruthenicumMurr. from Qinghai-Tibet Plateau

机译：转录组分析为野生甘露甘露菊的果实发育提供了见解。 来自青藏高原

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摘要

Lycium ruthenicumMurr. is an important ecological and economic species in the Qaidam Basin of Qinghai-Tibet Plateau. Its black fruits (BF) are rich in anthocyanins, which have health-promoting properties for humans and thus provide nutritional benefits for this plant. Although the fruit quality of natural white fruit (WF) is affected by the disappearance of pigmentation in phenotypes, this phenomenon provides an opportunity to unravel the complex color metabolic networks. In this study, anthocyanin profiling confirmed that WF was formed due to anthocyanin loss. Transcriptome analysis of BF and WF revealed 101,466 unigenes, 261 of which were identified as the putative homologs of color-related genes in other species. Genes encoding the enzymes involved in flavonoid biosynthesis were also identified systematically. The structural gene expression levels ofchalcone synthase(CHS),chalcone isomerase(CHI),flavonoid 3 ' 5 '-hydroxylase(F3 ' 5 ' H),dihydroflavonol 4-reductase(DFR),anthocyanidin synthase(ANS), andanthocyanidin 3-O-glucosyltransferase(UFGT) were highly similar and significantly positively correlated with anthocyanin accumulation rate in BF. In particular,F3 ' 5 ' H,UFGT,ANS, andDFRexpression levels in BF were 2391, 119, 96, and 85 times higher than those in WF at S3 (35 days after anthesis), respectively. This result strongly suggests that the low expression of these genes in WF is responsible for the anthocyanin loss. Meanwhile, the expression patterns of the anthocyanin regulatory genes were also investigated by qRT-PCR. Mass sequencing data were obtained and annotated by deep sequencing and provided a platform for future function and molecular biological research onL. ruthenicumMurr.
机译：黑枸杞。是青藏高原柴达木盆地重要的生态经济物种。它的黑色果实（BF）富含花青素，对人体有促进健康的作用，因此为这种植物提供营养益处。虽然天然白果（WF）的果实质量受到表型中色素沉着消失的影响，但这一现象为解开复杂的颜色代谢网络提供了机会。在这项研究中，花青素分析证实，WF是由于花青素损失而形成的。BF和WF的转录组分析显示有101466个单基因，其中261个被鉴定为其他物种颜色相关基因的假定同源物。编码类黄酮生物合成相关酶的基因也得到了系统鉴定。查尔酮合酶（CHS）、查尔酮异构酶（CHI）、类黄酮3'5'羟化酶（F3'5'H）、二氢黄酮醇4-还原酶（DFR）、花青素合酶（ANS）和花青素3-O-葡萄糖基转移酶（UFGT）的结构基因表达水平高度相似，并与BF中花青素的积累率显著正相关。特别是，在S3（开花后35天），BF中的F3'5'H、UFGT、ANS和DFREX表达水平分别是WF中的2391、119、96和85倍。这一结果有力地表明，这些基因在WF中的低表达是花青素损失的原因。同时，利用qRT-PCR技术研究了花青素调控基因的表达模式。通过深度测序获得大量测序数据并进行注释，为未来的功能和分子生物学研究提供了平台。露丝·坎穆尔。

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