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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Physio-biochemical and molecular assessment of Iron (Fe2+) toxicity responses in contrasting indigenous aromatic Joha rice cultivars of Assam, India
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Physio-biochemical and molecular assessment of Iron (Fe2+) toxicity responses in contrasting indigenous aromatic Joha rice cultivars of Assam, India

机译：铁（Fe2 +）毒性反应对比土着芳香族约翰稻米稻草，印度的毒性反应的生理生化和分子评估

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摘要

Iron (Fe) toxicity is one of the major abiotic stresses which limits the yield of lowland rice. This study aims to investigate the physiological, biochemical, and molecular aspects of two contrasting aromatic Joha rice, viz., Keteki and Kola Joha of Assam. Oxidative damage caused due to Fe(2+)toxicity was quantitatively determined. Fe(2+)toxicity in the growth medium increases the level of ROS and anti-oxidative enzyme activity. Along with the aforementioned damage caused due to Fe(2+)toxicity, chlorophyll content decreases in both the rice varieties. Detection of Fe(3+)and Fe(2+)was also conducted by Perls' Prussian and Turnbull blue method, respectively. In addition, spectrophotometric quantification of Fe(2+)was determined by 2, 2 '-Bipyridyl (Bpy). Above 2.5 mM, Fe(2+)toxicity was found to be lethal in rice seedlings affecting their total growth and biomass. Gene expression analysis of iron-regulated transporter 1 (OsIRT1), Yellow Stripe-Like 15 (OsYSL15), and ferritin 1 (OsFer1) revealed the differential gene expression over a time period of Fe(2+)toxicity. Our study suggested that the different parameters which are considered here can be helpful for the better understanding of how aromatic Joha rice performed under Fe(2+)toxicity which can also help to reveal broader aspects that how gene players are involved in the iron homeostasis mechanism in Joha rice in coming future.
机译：铁毒性是限制低地水稻产量的主要非生物胁迫之一。本研究旨在研究两种截然不同的芳香乔哈大米的生理、生化和分子方面。，阿萨姆邦的凯特基和科拉·乔哈。定量测定了Fe（2+）毒性引起的氧化损伤。生长培养基中的Fe（2+）毒性增加了ROS水平和抗氧化酶活性。除了上述由Fe（2+）毒性造成的损害外，两个水稻品种的叶绿素含量都降低。此外，还分别采用波尔斯普鲁士法和特恩布尔蓝法检测铁（3+）和铁（2+）的含量。此外，通过2，2'-联吡啶（Bpy）测定铁（2+）的分光光度定量。在2.5mm以上，Fe（2+）对水稻幼苗具有致死性，影响其总生长和生物量。铁调节转运体1（OsIRT1）、黄条样15（OsYSL15）和铁蛋白1（OsFer1）的基因表达分析揭示了铁（2+）毒性在一段时间内的差异基因表达。我们的研究表明，这里考虑的不同参数有助于更好地理解芳香乔哈大米在Fe（2+）毒性下的表现，这也有助于揭示未来乔哈大米铁稳态机制中基因参与者的更广泛方面。

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