Discovering potential Streptomyces hormone producers by using disruptants of essential biosynthetic genes as indicator strains

Nguyen B Thao; Kitani Shigeru; Nitta Hiroko; Tomioka Toshiya; Nihira Takuya

首页> 外文期刊>The Journal of Antibiotics: An International Journal >Discovering potential Streptomyces hormone producers by using disruptants of essential biosynthetic genes as indicator strains

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Discovering potential Streptomyces hormone producers by using disruptants of essential biosynthetic genes as indicator strains

机译：通过使用必需的生物合成基因的破坏剂作为指示菌株来发现潜在的链霉菌生产者

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Autoregulators are low-molecular-weight signaling compounds that control the production of many secondary metabolites in actinomycetes and have been referred to as 'Streptomyces hormones'. Here, potential producers of Streptomyces hormones were investigated in 40 Streptomyces and 11 endophytic actinomycetes. Production of gamma-butyrolactone-type (IM-2, VB) and butenolide-type (avenolide) Streptomyces hormones was screened using Streptomyces lavendulae FRI-5 (Delta farX), Streptomyces virginiae (Delta barX) and Streptomyces avermitilis (Delta aco), respectively. In these strains, essential biosynthetic genes for Streptomyces hormones were disrupted, enabling them to respond solely to the externally added hormones. The results showed that 20% of each of the investigated strains produced IM-2 and VB, confirming that y-butyrolactone-type Streptomyces hormones are the most common in actinomycetes. Unlike the gamma-butyrolactone type, butenolide-type Streptomyces hormones have been discovered in recent years, but their distribution has been unclear. Our finding that 24% of actinomycetes (12 of 51 strains) showed avenolide activity revealed for the first time that the butenolide-type Streptomyces hormone is also common in actinomycetes.

机译：自动调节器是一种低分子量的信号化合物，控制放线菌中许多次级代谢产物的产生，被称为“链霉菌激素”。本文对40株链霉菌和11株内生放线菌中链霉菌激素的潜在产生菌进行了研究。分别使用薰衣草链霉菌FRI-5（Delta farX）、弗吉尼亚链霉菌（Delta barX）和阿维米特链霉菌（Delta aco）筛选γ-丁内酯型（IM-2，VB）和丁烯内酯型（avenolide）链霉菌激素的产生。在这些菌株中，链霉菌激素的基本生物合成基因被破坏，使它们能够仅对外部添加的激素作出反应。结果表明，20%的被调查菌株产生IM-2和VB，证实y-丁内酯型链霉菌激素在放线菌中最常见。与γ-丁内酯型不同，近年来发现了丁烯内酯型链霉菌激素，但其分布尚不清楚。我们的研究发现，24%的放线菌（51株中的12株）表现出avenolide活性，这首次表明丁烯内酯型链霉菌激素在放线菌中也很常见。

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《The Journal of Antibiotics: An International Journal》 |2017年第10期|共5页
作者
Nguyen B Thao; Kitani Shigeru; Nitta Hiroko; Tomioka Toshiya; Nihira Takuya;
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Osaka Univ Int Ctr Biotechnol 2-1 Yamadaoka Suita Osaka 5650871 Japan;

Osaka Univ Int Ctr Biotechnol 2-1 Yamadaoka Suita Osaka 5650871 Japan;

Osaka Univ Int Ctr Biotechnol 2-1 Yamadaoka Suita Osaka 5650871 Japan;

Osaka Univ Int Ctr Biotechnol 2-1 Yamadaoka Suita Osaka 5650871 Japan;

Osaka Univ Int Ctr Biotechnol 2-1 Yamadaoka Suita Osaka 5650871 Japan;

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Discovering potential Streptomyces hormone producers by using disruptants of essential biosynthetic genes as indicator strains

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