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首页> 外文期刊>The Journal of dermatology >Circulating tumor necrosis factor-alpha DNA are elevated in psoriasis
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Circulating tumor necrosis factor-alpha DNA are elevated in psoriasis

机译：循环肿瘤坏死因子-αDNA在牛皮癣中升高

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摘要

In psoriasis, tumor necrosis factor (TNF)-alpha is a key pro-inflammatory cytokine that activates keratinocytes to produce other inflammatory mediators. In addition, increased serum or plasma TNF-alpha levels are considered to be biomarkers of psoriasis. Circulating cell-free DNA (cfDNA) originates from apoptotic or necrotic cells and reflects the severity of cellular damage. Although cfDNA has recently attracted attention as a marker in the diagnosis and prognosis of various disorders, there are few reports of its clinical implications in the field of dermatology including psoriasis. The aim of this study was to investigate whether the TNF-alpha gene is present in the cfDNA, and whether its levels can be utilized as a biomarker for patients with psoriasis. cfDNA was isolated from serum samples of 79 patients with psoriasis vulgaris and 29 with psoriatic arthritis. The levels of TNF-alpha in the cfDNA were assessed by droplet digital polymerase chain reaction. In this study, we made two novel findings. First, circulating TNF-alpha DNA levels in the cfDNA were significantly higher in patients with psoriasis than in healthy controls. In addition, the area under the curve was 0.91, suggesting that serum TNF-alpha DNA levels are effective as a diagnostic biomarker. Second, the levels of TNF-alpha DNA copies in the cfDNA were positively correlated with the Psoriasis Area and Severity Index (PASI) score in the group of patients with a PASI score higher than 10. Generally, a PASI score of more than 10 is defined as severe psoriasis; therefore, the levels of TNF-alpha DNA copies in the cfDNA could be a biomarker for severity in patients with severe psoriasis. Further studies are needed to establish serum TNF-alpha DNA levels as a novel biomarker of psoriasis.
机译：在银屑病中，肿瘤坏死因子（TNF）-α是一种关键的促炎症细胞因子，可激活角质形成细胞产生其他炎症介质。此外，血清或血浆TNF-α水平升高被认为是银屑病的生物标志物。循环无细胞DNA（cfDNA）来源于凋亡或坏死细胞，反映细胞损伤的严重程度。尽管cfDNA最近作为一种标志物在各种疾病的诊断和预后方面引起了关注，但在包括银屑病在内的皮肤病领域，关于其临床意义的报道很少。本研究的目的是调查cfDNA中是否存在TNF-α基因，以及其水平是否可以用作银屑病患者的生物标志物。从79例寻常型银屑病和29例银屑病关节炎患者的血清样本中分离到cfDNA。通过液滴数字聚合酶链反应评估cfDNA中TNF-α的水平。在这项研究中，我们有两个新发现。首先，银屑病患者cfDNA中循环TNF-αDNA水平显著高于健康对照组。此外，曲线下面积为0.91，表明血清TNF-αDNA水平作为诊断生物标志物是有效的。其次，在PASI评分高于10的患者组中，cfDNA中TNF-αDNA拷贝的水平与银屑病面积和严重程度指数（PASI）评分呈正相关。一般来说，PASI评分超过10分被定义为严重银屑病；因此，cfDNA中TNF-αDNA拷贝的水平可能是严重银屑病患者严重程度的生物标志物。需要进一步的研究来确定血清TNF-αDNA水平作为银屑病的新生物标志物。

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