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首页> 外文期刊>The Journal of Nutritional Biochemistry >EGCG inhibited bladder cancer SW780 cell proliferation and migration both in vitro and in vivo via down-regulation of NF-kappa B and MMP-9
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EGCG inhibited bladder cancer SW780 cell proliferation and migration both in vitro and in vivo via down-regulation of NF-kappa B and MMP-9

机译：EGCG通过NF-Kappa B和MMP-9的下调抑制膀胱癌SW780细胞增殖和迁移。

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摘要

Epigallocatechin-3-gallate (EGCG), the bioactive polyphenol in green tea, has been demonstrated to have various biological activities. Our study aims to investigate the antiproliferation and antimigration effects of EGCG against bladder cancer SW780 cells both in vitro and in vivo. Our results showed that treatment of EGCG resulted in significant inhibition of cell proliferation by induction of apoptosis, without obvious toxicity to normal bladder epithelium SV-HUC-1 cells. EGCG also inhibited SW780 cell migration and invasion at 25-100 pM. Western blot confirmed that EGCG induced apoptosis in SW780 cells by activation of caspases-8,-9 and-3, Bax, Bcl-2 and PARP. Besides, animal study demonstrated that EGCG [100 mg/kg, intraperitoneal (i.p.) injection daily for 3 weeks] decreased the tumor volume significantly in mice bearing SW780 tumors, as well as the tumor weight (decreased by 68.4%). In addition, EGCG down-regulated the expression of nuclear factor-kappa B (NF-kappa B) and matrix metalloproteinase (MMP)-9 in both protein and mRNA level in tumor and SW780 cells. When NF-kappa B was inhibited, EGCG showed no obvious effect in cell proliferation and migration. In conclusion, our study demonstrated that EGCG was effective in inhibition SW780 cell proliferation and migration, and presented first evidence that EGCG inhibited SW780 tumor growth by down-regulation of NF-kappa B and MMP-9. (C) 2016 Elsevier Inc. All rights reserved.
机译：表没食子儿茶素没食子酸酯（EGCG）是绿茶中具有生物活性的多酚，已被证明具有多种生物活性。本研究旨在研究EGCG在体外和体内对膀胱癌SW780细胞的抗增殖和抗迁移作用。我们的研究结果表明，EGCG通过诱导凋亡显著抑制细胞增殖，对正常膀胱上皮SV-HUC-1细胞无明显毒性。EGCG在25-100时也能抑制SW780细胞的迁移和侵袭。westernblot证实EGCG通过激活caspases-8、-9和-3、Bax、Bcl-2和PARP诱导SW780细胞凋亡。此外，动物研究表明，EGCG[100 mg/kg，每日腹腔注射（持续3周）]可显著降低SW780荷瘤小鼠的肿瘤体积和肿瘤重量（减少68.4%）。此外，EGCG在肿瘤和SW780细胞的蛋白质和mRNA水平下调核因子-κB（NF-κB）和基质金属蛋白酶（MMP）-9的表达。当NF-κB受到抑制时，EGCG对细胞增殖和迁移没有明显影响。总之，我们的研究表明EGCG能有效抑制SW780细胞的增殖和迁移，并首次证明EGCG通过下调NF-κB和MMP-9抑制SW780肿瘤的生长。（C） 2016爱思唯尔公司版权所有。

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