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首页> 外文期刊>The Journal of Nutritional Biochemistry >The combined effect of pomegranate extract and tangeretin on the DMBA-induced breast cancer model
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The combined effect of pomegranate extract and tangeretin on the DMBA-induced breast cancer model

机译：石榴提取物和橘子对DMBA诱导乳腺癌模型的综合作用

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The aim of this study was to investigate the protective effects of pomegranate extract and tangeretin alone or in combination in DMBA-induced rat breast cancer model. A total of 68 female rats were randomly divided into 8 groups. The first 4 groups were designed as controls for cancer and treatment groups, and the control groups were composed of only control (C), Pomegranate (P), Tangeretin (T), and Pomegranate +Tangeretin (P + T) groups. The other four groups were designed as cancer and treatment groups and were composed of DMBA (D) and DMBA + Pomegranate (D + P), DMBA +Tangeretin (D + T), DMBA + Pomegranate +Tangeretin (D + P + T) groups. Tumor markers and angiogenesis parameters were studied from plasma samples obtained from rats. Histopathological, immunohistochemical, and TUNEL analyses and expressions of proteins affecting apoptosis and cell cycle were determined in breast tissue samples. In the DMBA group, plasma CA15-3, CEA, VEGF, MMP-9, and NF KB levels were significantly increased compared to the controls, but significant decreases were observed in these parameters except MMP-9 in the treatment groups. It was observed that p53 and Bax expressions significantly increased in both D + P and D + P + T groups compared to the DMBA group, and these findings were supported by Tunel and immunohistochemical findings. Cyclin D1 expressions were found to be significantly decreased only in the D + T group and supported by TUNEL and immunohistochemical findings. Immunohistochemical ER-alpha and Ki-67 immune reactivities were significantly decreased in all treatment groups compared to the DMBA group. Our results showed that combined application of pomegranate extract and tangeretin may be more beneficial in preventing breast cancer development. 0 2020 Elsevier Inc. All rights reserved.
机译：本研究的目的是研究石榴提取物和糖皮质激素单独或联合应用对DMBA诱导的大鼠乳腺癌模型的保护作用。将68只雌性大鼠随机分为8组。前4组被设计为癌症对照组和治疗组，对照组仅由对照组（C）、石榴组（P）、丹格瑞汀组（T）和石榴+丹格瑞汀组（P+T）组成。其他四组被设计为癌症和治疗组，由DMBA（D）和DMBA+石榴（D+P）、DMBA+丹格瑞汀（D+T）、DMBA+石榴+丹格瑞汀（D+P+T）组组成。从大鼠血浆样本中研究肿瘤标记物和血管生成参数。对乳腺组织样本进行组织病理学、免疫组织化学和TUNEL分析，并检测影响细胞凋亡和细胞周期的蛋白表达。与对照组相比，DMBA组的血浆CA15-3、CEA、VEGF、MMP-9和NF-KB水平显著升高，但除MMP-9外，治疗组的这些参数均显著降低。与DMBA组相比，D+P组和D+P+T组的p53和Bax表达均显著增加，这些结果得到了Tunel和免疫组化结果的支持。细胞周期蛋白D1的表达仅在D+T组显著降低，TUNEL和免疫组化结果支持了这一点。与DMBA组相比，所有治疗组的免疫组织化学ER-α和Ki-67免疫反应性均显著降低。我们的研究结果表明，联合应用石榴提取物和tangeretin可能更有利于预防乳腺癌的发展。2020爱思唯尔公司版权所有。

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