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首页> 外文期刊>The protein journal >New Synthetic Peptides Conjugated to Gold Nanoclusters: Antibiotic Activity Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)
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New Synthetic Peptides Conjugated to Gold Nanoclusters: Antibiotic Activity Against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

机译：缀合金纳米蛋白的新合成肽：针对大肠杆菌O157的抗生素活性：H7和耐甲氧西林金黄色葡萄球菌（MRSA）

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摘要

Gold nanoclusters protected with bovine serum albumin (AuNC) can be used in multiple biomedical applications through functionalization with two new and bioactive peptides. Both cationic peptides sequences of 17 amino acids in length and the cysteine residue at its C-terminus were designed and synthesized. Peptides were obtained by solid phase synthesis using the Fmoc strategy. Peptides may be coupled via disulfide bonds to AuNC with hydrodynamic size similar to 2 nm +/- 0.3 determined by dynamic light scattering and it had a zeta potential value equal to - 42 mV. Peptides named NBC2253 and NBC2254 were attached to the AuNC using N-succinimidyl-3-(2-pyridyl-dithiol) propionate as crosslinker agent. AuNC@NBC2253 was more active against methicillin-resistant Staphylococcus aureus (MIC50 6.5 mu M) and AuNC@NBC2254 exhibited higher antimicrobial activity than the free peptides on Escherichia coli O157:H7 (MIC50 3.5 mu M). No hemolysis was detected for any of the peptides. It is evidenced that these antimicrobial peptides conjugated to AuNC serve as promising agents to combat some multi-resistant bacterial strains and that the specific binding of these antimicrobial peptides to gold nanoclusters improves the interaction of these nanostructured systems with the biological target.
机译：用牛血清白蛋白（AuNC）保护的金纳米团簇通过两种新的生物活性肽的功能化可用于多种生物医学应用。设计并合成了长度为17个氨基酸的阳离子肽序列及其C端的半胱氨酸残基。利用Fmoc策略通过固相合成获得肽。肽可以通过二硫键与AuNC偶联，其流体动力学尺寸类似于动态光散射测定的2 nm+/-0.3，其zeta电位值等于-42 mV。使用N-琥珀酰亚胺-3-（2-吡啶基二硫醇）丙酸盐作为交联剂，将名为NBC2253和NBC2254的肽连接到AuNC上。AuNC@NBC2253对耐甲氧西林金黄色葡萄球菌（MIC50 6.5μM）和AuNC@NBC2254在大肠杆菌O157:H7（MIC50 3.5μM）上表现出比游离肽更高的抗菌活性。未检测到任何肽的溶血。有证据表明，这些与AuNC结合的抗菌肽是对抗某些多重耐药菌株的有前途的药物，并且这些抗菌肽与金纳米簇的特异性结合改善了这些纳米结构系统与生物靶标的相互作用。

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  • 来源
    《The protein journal》 |2019年第5期|共9页
  • 作者

    Prada Y. A.; Guzman Fanny; Ortiz Claudia; Cabanzo Rafael; Torres Rodrigo; Mejia-Ospino Enrique;

  • 作者单位

    Univ Ind Santander CMN LEAM Parque Tecnol Guatiguara Piedecuesta 681012 Colombia;

    Pontificia Univ Catolica Valparaiso NBC Lab Sintesis Peptidos Valparaiso 2373223 Chile;

    Univ Ind Santander Fac Ciencias Escuela Quim Grp Invest Bioquim Microbiol GIBIM Bucaramanga;

    Univ Ind Santander CMN LEAM Parque Tecnol Guatiguara Piedecuesta 681012 Colombia;

    Univ Ind Santander Fac Ciencias Escuela Quim Grp Invest Bioquim Microbiol GIBIM Bucaramanga;

    Univ Ind Santander CMN LEAM Parque Tecnol Guatiguara Piedecuesta 681012 Colombia;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 蛋白质;
  • 关键词

    Gold nanoclusters; Antimicrobial peptides; E; coli O157; H7; MRSA;

    机译：金纳米蛋白;抗微生物肽;e;大肠杆菌o157;h7;mrsa;

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