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首页> 外文期刊>The protein journal >Variation in the Protein Composition and Biological Activity of King Cobra (Ophiophagus hannah) Venoms
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Variation in the Protein Composition and Biological Activity of King Cobra (Ophiophagus hannah) Venoms

机译：眼镜蛇（Ophiophagus Hannah）毒液的蛋白质组成和生物活性的变异

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The biochemical properties and biological activities of the venom from three individual Ophiophagus hannah (King cobra) specimens was compared. The toxicity against mice, the cytotoxicity against five cell lines, and the antioxidant activity were measured. The KV2 venom showed a higher cytotoxicity than the KV6 and the non-cytotoxic KV9 venoms. Comparative analysis of the O. hannah venom proteins was performed after 2-dimensional (2-D) denaturing gel electrophoresis and reverse phase high performance liquid chromatography (RP-HPLC). 2-D analysis by isoelectric focusing (IEF) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) resolution of the venoms revealed significant differences between all three venoms, with most spots being unique to that venom. Only 2 out of the 13-16 distinct spots were common to all three venoms, and four spots were common to KV6 and KV9. KV2 had the highest proportion of low molecular mass spots, and KV6 and KV9 appeared more related to each other than to KV9. From peptide mass mapping by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and MASCOT-based amino acid sequence database searching, the two venom proteins that were common to all three specimens are likely to be ophanin and acidic phospholipase A(2) (PLA(2)), whilst the proteins unique to the cytotoxic KV2 venom, included three other PLA(2) proteins. The RP-HPLC pattern of KV2 was different from the other two venoms with a higher protein concentration eluting in the 31-41% (v/v) acetonitrile (ACN) fraction than for the other two venoms.
机译：比较了三种眼镜王蛇毒液的生化特性和生物活性。测定其对小鼠的毒性、对五种细胞系的细胞毒性和抗氧化活性。KV2毒液的细胞毒性高于KV6和非细胞毒性KV9毒液。在二维（2-D）变性凝胶电泳和反相高效液相色谱（RP-HPLC）后，对O.hannah毒液蛋白质进行比较分析。通过等电聚焦（IEF）十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）对毒液进行二维分析，发现三种毒液之间存在显著差异，大多数斑点是该毒液特有的。在13-16个不同的斑点中，只有2个是所有三种毒液共有的，4个斑点是KV6和KV9共有的。KV2的低分子质量斑点比例最高，KV6和KV9之间的相关性似乎比KV9更大。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的肽质谱图和基于吉祥物的氨基酸序列数据库搜索，所有三个样本共有的两种毒液蛋白质可能是ophanin和酸性磷脂酶A（2）（PLA（2）），而细胞毒性KV2毒液特有的蛋白质包括其他三种PLA（2）蛋白质。KV2的RP-HPLC模式与其他两种毒液不同，在31-41%（v/v）乙腈（ACN）部分中洗脱的蛋白质浓度高于其他两种毒液。

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