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首页> 外文期刊>Tropical Animal Health and Production >The use of PCR technique in the identification of Mycobacterium species responsible for bovine tuberculosis in cattle and buffaloes in Pakistan
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The use of PCR technique in the identification of Mycobacterium species responsible for bovine tuberculosis in cattle and buffaloes in Pakistan

机译：PCR技术在巴基斯坦牛和水牛牛结核中鉴定的分枝杆菌物种的鉴定

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摘要

Bovine tuberculosis is one of the important diseases of dairy and wild animals. The disease is prevalent all over the world, though developed countries have tremendously reduced the prevalence through eradication campaigns. The prevalence of disease in Pakistan on the basis of tuberculin testing or culture isolation of the organism has been reported previously. It is, however, important to use the latest diagnostic tools, i.e. PCR to confirm the type of Mycobacterium infecting the animals in Pakistan. Therefore, the present study was carried out to assess the utility of direct PCR on milk samples and nasal swabs to confirm the type of Mycobacterium infecting the animals. This study was carried out on 215 cattle and buffaloes of more than 2 years of age present at two livestock farms. The tuberculin results showed 22.5 % prevalence at one farm and 25.9 % at the other with an overall prevalence of 24.7 %. The 92.5 % of milk samples and/or nasal swabs showed positive PCR for Mycobacterium genus, 86.8 % for Mycobacterium tuberculosis complex and 77.4 % for Mycobacterium bovis. The M. bovis by PCR was detected in 13.2 % of milk samples, 24.5 % of nasal swabs and 39.6 % of both milk samples + nasal swabs. The results suggested that there are 60 % higher chance for a nasal swab to yield a positive PCR for M. bovis than the milk sample. It can be concluded from the present study that tuberculin testing is a useful method in studying the prevalence of disease as the PCR for Mycobacterium genus was positive in 92.5 %, M. tuberculosis complex in 86.8 % and Mycobacterium bovis in 77.4 % cases.
机译：牛结核病是奶牛和野生动物的重要疾病之一。这种疾病在全世界都很流行，尽管发达国家通过根除运动极大地降低了患病率。根据结核菌素试验或细菌培养分离，巴基斯坦的疾病患病率此前已有报道。然而，重要的是使用最新的诊断工具，即PCR来确认感染巴基斯坦动物的分枝杆菌类型。因此，本研究旨在评估直接PCR对牛奶样本和鼻拭子的效用，以确认感染动物的分枝杆菌类型。这项研究是在两个畜牧场的215头2岁以上的牛和水牛上进行的。结核菌素结果显示，一个农场的患病率为22.5%，另一个农场的患病率为25.9%，总体患病率为24.7%。92.5%的牛奶样本和/或鼻拭子显示分枝杆菌属PCR阳性，86.8%的结核分枝杆菌复合物PCR阳性，77.4%的牛分枝杆菌PCR阳性。通过PCR在13.2%的牛奶样品、24.5%的鼻拭子和39.6%的牛奶样品+鼻拭子中检测到牛分枝杆菌。结果表明，鼻拭子产生牛分枝杆菌阳性PCR的几率比牛奶样本高60%。从本研究可以得出结论，结核菌素试验是研究疾病流行的一种有用方法，因为结核分枝杆菌属PCR阳性率为92.5%，结核分枝杆菌复合物阳性率为86.8%，牛分枝杆菌阳性率为77.4%。

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