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首页> 外文期刊>Human and Experimental Toxicology >Gene expression in rat placenta after exposure to di(2-ethylhexyl) phthalate
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Gene expression in rat placenta after exposure to di(2-ethylhexyl) phthalate

机译：在暴露于DI（2-乙基己基）邻苯二甲酸酯后大鼠胎盘的基因表达

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摘要

The organic compound di(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in many products. Exposure to DEHP has been reported to lead to adverse pregnancy outcomes by suppressing placenta growth and development. The aim of this study was to determine the gene expression profiles of rat placenta exposed to (DEHP) and identify genes crucial for the DEHP response. Three groups of Wistar rats were administered an intragastric dose of 1,000 mg/kg DEHP, 500 mg/kg DEHP, or corn oil, RNA was isolated from placenta tissue, and hybridization was performed. Gene expression profiles were analyzed by identifying functional enrichment, differentially expressed genes (DEGs), protein–protein interaction (PPI) networks and modules, and transcription factor (TF)-miRNA-target regulatory networks. We obtained 2,032 DEGs, including cytochrome P450, family 2, subfamily R, polypeptide 1 (CYP2R1), sterol O-acyltransferase 2 (SOAT2), and 24-dehydrocholesterol reductase (DHCR24) from the steroid biosynthesis pathway and somatostatin receptor 4 (SSTR4) and somatostatin receptor 2 (SSTR2) in the neuroactive ligand-receptor interaction pathway. The PPI network included 476 nodes, 2,682 interaction pairs, and three sub-network modules. Moreover, eight miRNAs, three TFs, and 176 regulatory pairs were obtained from the TF-miRNA-target regulatory network. CYP2R1, SOAT2, DHCR24, SSTR4, and SSTR2 may affect DEHP influence on rat placenta development.
机译：有机化合物邻苯二甲酸二（2-乙基己基）酯（DEHP）在许多产品中广泛用作增塑剂。据报道，接触DEHP会抑制胎盘的生长和发育，从而导致不良妊娠结局。本研究的目的是确定暴露于DEHP的大鼠胎盘的基因表达谱，并确定对DEHP反应至关重要的基因。三组Wistar大鼠灌胃给予1000 mg/kg DEHP、500 mg/kg DEHP或玉米油，从胎盘组织中分离RNA，并进行杂交。通过鉴定功能富集、差异表达基因（DEG）、蛋白质-蛋白质相互作用（PPI）网络和模块，以及转录因子（TF）-miRNA靶调控网络，分析基因表达谱。我们从类固醇生物合成途径和神经活性配体-受体相互作用途径中获得了2032个DEG，包括细胞色素P450、家族2、亚家族R、多肽1（CYP2R1）、甾醇O-酰基转移酶2（SOAT2）和24-脱氢胆固醇还原酶（DHCR24）。PPI网络包括476个节点、2682个交互对和三个子网络模块。此外，从TF-miRNA靶调控网络中获得了8个miRNA、3个TF和176个调控对。CYP2R1、SOAT2、DHCR24、SSTR4和SSTR2可能影响DEHP对大鼠胎盘发育的影响。

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