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首页> 外文期刊>Developmental dynamics: an official publication of the American Association of Anatomists >Cell lineage- and expression-based inference of the roles of forkhead box transcription factor Foxc2 in craniofacial development
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Cell lineage- and expression-based inference of the roles of forkhead box transcription factor Foxc2 in craniofacial development

机译：基于细胞谱系和基于表达的颅骨箱转录因子FoxC2在颅面发育中的作用推断

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Background: Foxc2 is a member of the winged helix/forkhead (Fox) box family of transcription factors. Loss of function of Foxc2 causes craniofacial abnormalities such as cleft palate and deformed cranial base, but its role during craniofacial development remains to be elucidated. Results: The contributions of Foxc2-positive and its descendant cells to the craniofacial structure at E18.5 were examined using a tamoxifen-inducible Cre driver mouse (Foxc2-CreERT2) crossed with the R26R-LacZ reporter mouse. Foxc2 expression at E8.5 is restricted to the cranial mesenchyme, contributing to specific components including the cranial base, sensory capsule, tongue, upper incisor, and middle ear. Expression at E10.5 was still positively regulated in most of those regions. In situ hybridization analysis of Foxc2 and its closely related gene, Foxc1, revealed that expression domains of these genes largely overlap in the cephalic mesenchyme. Meanwhile, the tongue expressed Foxc2 but not Foxc1, and its development was affected by the neural crest-specific deletion of Foxc2 in mice (Wnt1-Cre; Foxc2~fl/fl). Conclusions: Foxc2 is expressed in cranial mesenchyme that contributes to specific craniofacial tissue components from an early stage, and it seems to be involved in their development in cooperation with Foxc1. Foxc2 also has its own role in tongue development.
机译：背景：Foxc2是有翼螺旋/叉头（Fox）盒转录因子家族的成员。Foxc2功能丧失会导致颅面畸形，如腭裂和颅底畸形，但其在颅面发育中的作用仍有待阐明。结果：在E18，Foxc2阳性细胞及其后代细胞对颅面结构的贡献。使用三苯氧胺诱导的Cre驱动小鼠（Foxc2-CreERT2）与R26R LacZ报告小鼠杂交，对5只小鼠进行检测。E8的Foxc2表达。5仅限于颅骨间质，构成颅底、感觉囊、舌头、上门牙和中耳等特定成分。E10的表达。在这些地区中的大多数地区，5%仍然受到积极监管。Foxc2及其密切相关基因Foxc1的原位杂交分析显示，这些基因的表达域在头间充质中大部分重叠。同时，舌头表达Foxc2，但不表达Foxc1，其发育受小鼠Foxc2神经嵴特异性缺失（Wnt1-Cre；Foxc2~fl/fl）的影响。结论：Foxc2在颅骨间质中表达，从早期起就参与了特定的颅面组织成分，并且似乎与Foxc1共同参与了它们的发育。Foxc2在舌头发育中也有自己的作用。

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