...
首页> 外文期刊>Domestic Animal Endocrinology >Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep
【24h】

Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep

机译:外源性孕激素在绵羊中差异地改变了未成熟巨核盐酸络合物的基因表达

获取原文
获取原文并翻译 | 示例
           

摘要

This study evaluated the role of progesterone (P-4) and medroxyprogesterone acetate (MAP) on the molecular status of immature cumulus-oocyte complexes (COCs) and the implications for oocyte quality in sheep. The number of viable COCs per ewe and the rate of COCs screened for developmental competence by brilliant cresyl blue positive (BCB+) were similar (P > 0.05), respectively, across treatments (P-4: 7.7 +/- 0.7 and 4.7 +/- 1.2; MAP: 5.7 +/- 1.0 and 3.5 +/- 2.3; and control: 5.7 +/- 1.1 and 3.6 +/- 2.4). The COCs' gene expression was altered by exogenous progestogens compared with the control group: markers of steroidogenic pathway (FSH receptor [FSHr], LH receptor [LHr], and estradiol receptor alpha) and of quality (zygote arrest 1, growth differentiation factor 9, and B-cell lymphoma 2) were in abundance in P-4 (P < 0.05). In addition, reelin protein (RELN) was downregulated, and Bcl-2 was upregulated in MAP (P < 0.05). In the P-4 vs MAP comparison, FSHr, LHr, and RELN genes were upregulated (P < 0.05) in the P-4 group. In conclusion, P-4 and MAP promoted dissimilar effects on transcriptome profiling of immature BCB-selected COCs, possibly due to the differences in the chemical structure of progestogens and concentrations of serum P-4. Exogenous P-4 impacted positively on the profile of genes related to oocyte quality. (C) 2020 Published by Elsevier Inc.
机译:本研究评估了孕酮(P-4)和醋酸甲羟孕酮(MAP)对绵羊未成熟卵丘卵母细胞复合体(COCs)分子状态的作用以及对卵母细胞质量的影响。每只母羊的活COCs数量和用灿烂甲酚蓝阳性(BCB+)筛选发育能力的COCs比率在不同处理中分别相似(P>0.05)(P-4:7.7+/-0.7和4.7+/-1.2;MAP:5.7+/-1.0和3.5+/-2.3;对照:5.7+/-1.1和3.6+/-2.4)。与对照组相比,外源性孕激素改变了COCs的基因表达:类固醇生成途径(FSH受体[FSHr]、LH受体[LHr]和雌二醇受体α)和质量(合子阻滞1、生长分化因子9和B细胞淋巴瘤2)的标记物在P-4中大量存在(P<0.05)。此外,在MAP中,reelin蛋白(RELN)表达下调,Bcl-2表达上调(P<0.05)。在P-4与MAP的比较中,P-4组的FSHr、LHr和RELN基因上调(P<0.05)。综上所述,P-4和MAP促进了未成熟BCB选择的COC转录组分析的不同效果,可能是由于孕激素的化学结构和血清P-4浓度的差异。外源性P-4对卵母细胞质量相关基因的分布有积极影响。(C) 2020年由爱思唯尔公司出版。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号