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首页> 外文期刊>Domestic Animal Endocrinology >Association of GH polymorphisms with growth traits in buffaloes
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Association of GH polymorphisms with growth traits in buffaloes

机译：GH多态性与水牛生长特征的关联

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Members of the somatotrophic axis, especially GH and IGF1, are essential for growth. The association between GH polymorphisms and growth traits was numerously studied in cattle; however, no data are available for such association studies in buffalo. Therefore, this study was conducted to screen for polymorphisms in the GH gene and to study their putative association with growth traits in 200 Egyptian buffaloes. Polymerase chain reaction single-strand conformation polymorphism and sequencing were applied to look for polymorphisms in 3 loci spanning all exons and introns of buffalo GH. The C (MspI+) >T (MspI-) SNP in intron3, which is well known in cattle, was not detected in the examined buffaloes. However, 2 missense mutations were detected in exon5: one previously detected p.Leu153Val SNP, with very low frequencies for the mutant (Val) allele and one novel p.Asn174His SNP. At weaning age, the p.Leu153Val SNP was significantly associated with weaning body weight and gain with the positive effect of the wild allele (Leu) and higher GH serum, mRNA, and protein levels in animals with Leu/Asn and Leu/His haplotypes. At yearling age, the 2 SNPs associated with yearling weight and gain with positive effect for the mutant (Val and His) alleles with increased GH, and IGF1 serum, mRNA, and protein and GHR mRNA and protein levels in animals with Val/Asn haplotype. Therefore, the selection of Egyptian buffaloes with the Val/Asn haplotype could improve the growth traits of Egyptian buffaloes at yearling age which is the target age for perfect growing. (C) 2020 Elsevier Inc. All rights reserved.
机译：生长激素轴的成员，尤其是GH和IGF1，对生长至关重要。生长激素多态性与牛生长性状之间的关联已被大量研究；然而，在布法罗没有此类关联研究的数据。因此，本研究旨在筛选生长激素基因的多态性，并研究其与200头埃及水牛生长性状的可能关联。应用聚合酶链反应-单链构象多态性和测序技术，在水牛生长激素的所有外显子和内含子的3个位点上寻找多态性。牛中已知的内含子3中的C（MspI+）>T（MspI-）SNP在受检水牛中未检测到。然而，在外显子5中检测到2个错义突变：一个以前检测到的p.Leu153Val SNP，突变（Val）等位基因的频率非常低，一个新的p.Asn174His SNP。在断奶年龄，p.Leu153Val单核苷酸多态性与断奶体重和体重增加显著相关，野生等位基因（Leu）的积极作用以及Leu/Asn和Leu/His单倍型动物中较高的GH血清、mRNA和蛋白质水平。在一岁时，与一岁体重和体重增加相关的2个SNP对具有Val/Asn单倍型的动物中GH、IGF1血清、mRNA和蛋白质以及GHR mRNA和蛋白质水平增加的突变（Val和His）等位基因具有积极影响。因此，选择Val/Asn单倍型的埃及水牛可以改善埃及水牛在一岁时的生长特性，这是完美生长的目标年龄。（C） 2020爱思唯尔公司版权所有。

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