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首页> 外文期刊>Journal of applied toxicology >Total dose defines the incidence of percutaneous IgE/IgG1 mediated immediate-type hypersensitivity caused by papain
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Total dose defines the incidence of percutaneous IgE/IgG1 mediated immediate-type hypersensitivity caused by papain

机译：总剂量定义经皮IgE / IgG1的发病率，介导的木瓜蛋白酶引起的立即型过敏率

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摘要

Assessment of human health risk requires an understanding of antigen dose metrics associated with toxicity. Whereas assessment of the human health risk for delayed-type hypersensitivity is understood, the metrics remain unclear for percutaneous immediate-type hypersensitivity (ITH) mediated by IgE/IgG1. In this work, we aimed to investigate the dose metric for percutaneous ITH mediated by IgE/IgG1 responses. Papain, which causes ITH via percutaneous sensitization in humans, was used to sensitize guinea pigs and mice. The total dose per animal or dose per unit area was adjusted to understand the drivers of sensitization. Passive cutaneous anaphylaxis (PCA) and enzyme-linked immunosorbent assay (ELISA) for papain-specific IgG1 enabled quantification of the response in guinea pigs. In mice, the number of antigen-bearing B cells in the draining lymph nodes (DLN) was calculated using flow cytometry, papain-specific IgG1 and IgE levels were quantified by ELISA. PCA positive test rates and the amounts of antigen-specific antibody corresponded with total dose per animal, not dose per unit area. Furthermore, the number of B cells taking up antigen within DLN also correlated with total dose. These findings indicate that the total antigen dose is the important metric for percutaneous IgE/IgG1-mediated ITH.
机译：评估人类健康风险需要了解与毒性相关的抗原剂量指标。虽然对迟发性超敏反应的人类健康风险的评估尚不清楚，但IgE/IgG1介导的经皮即时型超敏反应（ITH）的指标仍不清楚。在这项工作中，我们旨在研究IgE/IgG1反应介导的经皮ITH的剂量计量。木瓜蛋白酶通过人体经皮致敏引起ITH，用于致敏豚鼠和小鼠。调整每只动物的总剂量或每单位面积的剂量，以了解致敏因素。被动皮肤过敏反应（PCA）和木瓜蛋白酶特异性IgG1的酶联免疫吸附试验（ELISA）可以量化豚鼠的反应。在小鼠中，使用流式细胞术计算引流淋巴结（DLN）中携带抗原的B细胞的数量，通过ELISA定量木瓜蛋白酶特异性IgG1和IgE水平。PCA阳性率和抗原特异性抗体的数量与每只动物的总剂量相对应，而不是每单位面积的剂量。此外，在DLN内吸收抗原的B细胞数量也与总剂量相关。这些发现表明，总抗原剂量是经皮IgE/IgG1介导的ITH的重要指标。

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