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首页> 外文期刊>Journal of applied toxicology >Comparison of cytotoxicity of Ag/ZnO and Ag@ZnO nanocomplexes to human umbilical vein endothelial cells in vitro
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Comparison of cytotoxicity of Ag/ZnO and Ag@ZnO nanocomplexes to human umbilical vein endothelial cells in vitro

机译：Ag / ZnO和Ag ZnO纳米复合对体外人脐静脉内皮细胞的细胞毒性的比较

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摘要

Novel metal and metal oxide-based nanocomplexes are being developed due to their superior properties compared with nanoparticles (NPs) based on single composition. In this study, we synthesized Ag-coated ZnO (Ag/ZnO) and Ag-doped ZnO (Ag@ZnO) NPs. The cytotoxicity and mechanisms associated with the synthesized NPs were investigated to understand the influence of Ag positions on biocompatibility of the NPs. After exposure to human umbilical vein endothelial cells (HUVECs), Ag/ZnO, Ag@ZnO, and ZnO NPs all significantly induced cytotoxicity, but the cytotoxic effects of Ag/ZnO and Ag@ZnO NPs were more modest in comparison with ZnO NPs. At cytotoxic concentrations, all NPs significantly induced intracellular Zn ions, which suggested a role of excessive Zn ions on cytotoxicity of NPs. All types of NPs significantly induced the expression of endoplasmic reticulum (ER) stress genes including DNA damage-inducible transcript 3 (DDIT3), X-box binding protein 1 (XBP-1), and ER to nucleus signaling 1 (ERN1), but Ag/ZnO and Ag@ZnO NPs were less effective to induce DDIT3 and XBP-1 expression compared with ZnO NPs. Not surprisingly, only ZnO NPs significantly induced the expression of caspase 3. Combined, the results from this study showed that Ag/ZnO and Ag@ZnO NPs were less cytotoxic and less potent to induce ER stress gene expression compared with ZnO NPs, but there were no significant differences between Ag/ZnO and Ag@ZnO NPs. Our results may provide novel understanding about the biocompatibility of Ag-ZnO nanocomplexes.
机译：与基于单一成分的纳米颗粒（NPs）相比，基于金属和金属氧化物的新型纳米复合物具有优越的性能，因此正在开发中。在本研究中，我们合成了银包覆的ZnO（Ag/ZnO）和银掺杂的ZnO(Ag@ZnO)核动力源。为了了解银位置对纳米颗粒生物相容性的影响，研究了与合成纳米颗粒相关的细胞毒性和机制。暴露于人脐静脉内皮细胞（HUVECs）、Ag/ZnO、，Ag@ZnO和ZnO NP均显著诱导细胞毒性，但Ag/ZnO和Ag@ZnO与氧化锌纳米颗粒相比，纳米颗粒更温和。在细胞毒性浓度下，所有NP均显著诱导细胞内锌离子，这表明过量锌离子对NP的细胞毒性有作用。所有类型的NP均显著诱导内质网（ER）应激基因的表达，包括DNA损伤诱导转录本3（DDIT3）、X盒结合蛋白1（XBP-1）和内质网至核信号1（ERN1），但Ag/ZnO和Ag@ZnO与ZnO NP相比，NP诱导DDIT3和XBP-1表达的效果较差。毫不奇怪，只有ZnO NP能显著诱导caspase 3的表达。这项研究的结果表明，银/氧化锌和Ag@ZnO与氧化锌纳米颗粒相比，纳米颗粒的细胞毒性较小，诱导内质网应激基因表达的能力也较低，但银/氧化锌纳米颗粒与氧化锌纳米颗粒之间没有显著差异Ag@ZnO核动力源。我们的结果可能为银-氧化锌纳米复合物的生物相容性提供新的理解。

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