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首页> 外文期刊>Journal of biochemical and molecular toxicology >Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms
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Novel polymeric microspheres: Synthesis, enzyme immobilization, antimutagenic activity, and antimicrobial evaluation against pathogenic microorganisms

机译：新型聚合物微球：合成，酶固定化，抗微生物的抗微生物评估

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摘要

New polymeric microspheres containing azomethine (1a-1c and 2a-2c) were synthesized by condensation to compare the enzymatic properties of the enzyme glucose oxidase (GOx) and to investigate antimutagenic and antimicrobial activities. The polymeric microspheres were characterized by elemental analysis, infrared spectra (FT-IR), proton nuclear magnetic resonance spectra, thermal gravimetric analysis, and scanning electron microscopy analysis. The catalytic activity of the glucose oxidase enzyme follows Michaelis-Menten kinetics. Influence of temperature, reusability, and storage capacity of the free and immobilized glucose oxidase enzyme were investigated. It is determined that immobilized enzymes exhibit good storage stability and reusability. After immobilization of GOx in polymeric supports, the thermal stability of the enzyme increased and the maximum reaction rate (V_max) decreased. The activity of the immobilized enzymes was preserved even after 5 months. The antibacterial and antifungal activity of the polymeric microspheres were evaluated by well-diffusion method against some selected pathogenic microorganisms. The antimutagenic properties of all compounds were also examined against sodium azide in human lymphocyte cells by micronuclei and sister chromatid exchange tests.
机译：通过缩合法合成了含甲亚胺（1a-1c和2a-2c）的新型聚合物微球，以比较葡萄糖氧化酶（GOx）的酶学性质，并研究其抗突变和抗菌活性。通过元素分析、红外光谱（FT-IR）、质子核磁共振光谱、热重分析和扫描电镜分析对聚合物微球进行了表征。葡萄糖氧化酶的催化活性遵循米氏动力学。研究了温度、可重复使用性和储存能力对游离和固定化葡萄糖氧化酶的影响。结果表明，固定化酶具有良好的贮存稳定性和可重复使用性。在聚合物载体中固定GOx后，酶的热稳定性增加，最大反应速率（V_max）降低。固定化酶的活性在5个月后仍能保持。采用井扩散法对聚合物微球的抗菌和抗真菌活性进行了评价。通过微核试验和姐妹染色单体交换试验，对所有化合物的抗突变特性进行了检测。

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