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首页> 外文期刊>Journal of biochemical and molecular toxicology >Vitamin C inhibits glycidamide-induced genotoxicity and apoptosis in Sertoli cells
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Vitamin C inhibits glycidamide-induced genotoxicity and apoptosis in Sertoli cells

机译：维生素C抑制糖酰胺诱导的血肽诱导的血管毒性和凋亡

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摘要

Exposure to the food contaminant acrylamide and its reactive epoxide metabolite glycidamide (GA) induces reactive oxygen species (ROS)-mediated oxidative stress and subsequent cellular death. Recent studies have revealed that the toxic effects of acrylamide may be due to GA, especially on male reproductive system cells. In this regard, it is important to determine the effects of GA on Sertoli cells, which are essential cells for the male reproductive system. Antioxidants should be consumed in sufficient quantities to minimise the effects of environmental pollutants. This study aimed to determine the direct toxic effects of GA and protective effects of vitamin C (VitC) against GA-induced damage in Sertoli cells by measuring cell viability, cytotoxicity, lipid peroxidation, ROS, antioxidant enzyme levels, apoptosis and DNA damage. Sertoli cells were exposed to GA for 24 hours at four different concentrations (ranging between 1 and 1000 μM) and in addition to these GA concentrations to VitC (50μM). The results of cytotoxicity markers, such as cell viability and lactate dehydrogenase (LDH) showed that GA significantly reduced cell viability and increased LDH levels. We also found that GA induced overproduction of in-tracellular ROS, increased lipid peroxidation in cellular membrane and triggered cell apoptosis and genotoxicity. In addition, VitC supplementation ameliorated the adverse effects of GA on Sertoli cells. Consequently, these findings suggest that GA may damage the cell function in Sertoli cells, depending on the concentration. Additionally, it was evidenced that VitC has an ameliorative effect on toxicity caused by GA.
机译：暴露于食品污染物丙烯酰胺及其活性环氧化物代谢物缩水甘油酰胺（GA）会诱导活性氧（ROS）介导的氧化应激和随后的细胞死亡。最近的研究表明，丙烯酰胺的毒性作用可能是由GA引起的，尤其是对男性生殖系统细胞。在这方面，确定GA对支持细胞的影响很重要，支持细胞是男性生殖系统的基本细胞。应摄入足够数量的抗氧化剂，以尽量减少环境污染物的影响。本研究旨在通过检测细胞活力、细胞毒性、脂质过氧化、活性氧、抗氧化酶水平、细胞凋亡和DNA损伤，确定GA的直接毒性作用和维生素C（VitC）对GA诱导的支持细胞损伤的保护作用。将支持细胞暴露于四种不同浓度（1至1000μM）的GA中24小时，并将这些浓度的GA添加到VitC（50μM）中。细胞毒性标记物，如细胞活力和乳酸脱氢酶（LDH）的结果表明，GA显著降低细胞活力并增加LDH水平。我们还发现GA诱导细胞内ROS的过度产生，增加细胞膜脂质过氧化，触发细胞凋亡和遗传毒性。此外，补充维生素C可以改善GA对支持细胞的不良影响。因此，这些发现表明，GA可能会损害支持细胞的细胞功能，具体取决于浓度。此外，有证据表明，维生素C对GA引起的毒性有改善作用。

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