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首页> 外文期刊>Journal of biochemical and molecular toxicology >Vinculin presents unfavorable prediction in ovarian cancer and prevents proliferation and migration of ovarian cancer cells
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Vinculin presents unfavorable prediction in ovarian cancer and prevents proliferation and migration of ovarian cancer cells

机译：Vinculin呈现出卵巢癌的不利预测，并防止卵巢癌细胞的增殖和迁移

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The influences of Vinculin on many cancers were blurry, including ovarian cancer. Thus, we concentrated on the efficient role of Vinculin in ovarian cancer and explored the potential mechanism(s). Expression of Vinculin in ovarian cancer tissues and cell lines was investigated by real-time polymerase chain reaction, im-munohistochemistry, and Western blot. The Kaplan-Meier manner with the logrank was performed to assess overall survival. We further evaluated the relations between Vinculin expression and clinicopathological features of ovarian cancer. Moreover, Vinculin was overexpressed or silenced by respectively transfection with pcDNA-Vinculin or small interfering (si-Vinculin) into human ovarian cancer cell line Caov3 or human ovarian epithelial cell line (HOEpiC). Thereafter, cell viability, cell apoptosis, and migration were checked by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide, flow cytometer, and scratch assay, respectively. Likewise, the apoptosis- and migration-related proteins were distinguished by Western blot. Compared to the nontumor tissues or HOEpiC cells, Vinculin was significantly lower expressed in the ovarian cancer tissues and cells. Furthermore, we found out that Vinculin was primarily distributed at the cell membrane and cytoplasm. Moreover, Vinculin was negatively associated with International Federation of Gynecology and Obstetrics stage, grade, and distant metastasis. Overexpression of Vinculin dramatically weakened cell viability and migration and stimulated apoptosis. Conversely, suppression of Vinculin showed opposite results. Vinculin presents unfavorable prediction in ovarian cancer and inhibits ovarian cancer proliferation and migration.
机译：Vinculin对许多癌症的影响是模糊的，包括卵巢癌。因此，我们专注于长春花素在卵巢癌中的有效作用，并探索其潜在机制。通过实时聚合酶链反应、免疫组化和westernblot研究长春花素在卵巢癌组织和细胞系中的表达。采用Kaplan-Meier法和logrank法评估总体生存率。我们进一步评估了Vinculin表达与卵巢癌临床病理特征之间的关系。此外，通过分别将pcDNA Vinculin或小干扰（si Vinculin）转染到人卵巢癌细胞系Caov3或人卵巢上皮细胞系（HOEpiC）中，Vinculin过度表达或沉默。此后，分别通过3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2-H-四唑溴化铵、流式细胞仪和划痕试验检查细胞活力、细胞凋亡和迁移。同样，凋亡和迁移相关蛋白也通过westernblot进行了区分。与非肿瘤组织或HOEpiC细胞相比，Vinculin在卵巢癌组织和细胞中的表达显著降低。此外，我们还发现Vinculin主要分布在细胞膜和细胞质中。此外，Vinculin与国际妇产科联合会的分期、分级和远处转移呈负相关。Vinculin的过度表达显著削弱了细胞活力和迁移，并刺激了细胞凋亡。相反，抑制Vinculin显示出相反的结果。Vinculin对卵巢癌有不利预测，并抑制卵巢癌的增殖和迁移。

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