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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Prevascularization of 3D printed bone scaffolds by bioactive hydrogels and cell co‐culture
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Prevascularization of 3D printed bone scaffolds by bioactive hydrogels and cell co‐culture

机译：通过生物活性水凝胶和细胞共同培养3D印刷骨支架的血糖化

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Abstract Vascularization is a fundamental prerequisite for large bone construct development and remains one of the main challenges of bone tissue engineering. Our current study presents the combination of 3D printing technique with a hydrogel‐based prevascularization strategy to generate prevascularized bone constructs. Human adipose derived mesenchymal stem cells (ADMSC) and human umbilical vein endothelial cells (HUVEC) were encapsulated within our bioactive hydrogels, and the effects of culture conditions on in vitro vascularization were determined. We further generated composite constructs by forming 3D printed polycaprolactone/hydroxyapatite scaffolds coated with cell‐laden hydrogels and determined how the co‐culture affected vascularization and osteogenesis. It was demonstrated that 3D co‐cultured ADMSC‐HUVEC generated capillary‐like networks within the porous 3D printed scaffold. The co‐culture systems promoted in vitro vascularization, but had no significant effects on osteogenesis. The prevascularized constructs were subcutaneously implanted into nude mice to evaluate the in vivo vascularization capacity and the functionality of engineered vessels. The hydrogel systems facilitated microvessel and lumen formation and promoted anastomosis of vascular networks of human origin with host murine vasculature. These findings demonstrate the potential of prevascularized 3D printed scaffolds with anatomical shape for the healing of larger bone defects. ? 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1788–1798, 2018.
机译：摘要血管化是大骨构建发展的基本前提，也是骨组织工程面临的主要挑战之一。我们目前的研究将3D打印技术与水凝胶预血管化策略相结合，以生成预血管化骨结构。将人脂肪间充质干细胞（ADMSC）和人脐静脉内皮细胞（HUVEC）包裹在我们的生物活性水凝胶中，并测定培养条件对体外血管化的影响。我们通过形成3D打印的聚己内酯/羟基磷灰石支架，并在支架上涂上载细胞水凝胶，进一步生成复合结构，并确定共培养如何影响血管化和成骨。研究表明，3D共培养的ADMSC-HUVEC在多孔3D打印支架内生成毛细血管样网络。共培养系统促进了体外血管化，但对成骨没有显著影响。将预血管化构建物皮下植入裸鼠体内，以评估体内血管化能力和工程血管的功能。水凝胶系统促进了微血管和管腔的形成，促进了人类起源的血管网络与宿主小鼠血管系统的吻合。这些发现证明了具有解剖形状的预血管化3D打印支架在愈合较大骨缺损方面的潜力？2017威利期刊有限公司《生物医学材料研究》第B部分：Appl Biomater，106B:1788–1798，2018年。

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