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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Quantitative assessment of dentine mineralization and tubule occlusion by NovaMin and stannous fluoride using serial block face scanning electron microscopy
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Quantitative assessment of dentine mineralization and tubule occlusion by NovaMin and stannous fluoride using serial block face scanning electron microscopy

机译：用连续块面扫描电子显微镜测量诺氨基林和氟化芳烃和氟化锡闭塞的定量评估

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摘要

Dentine hypersensitivity (DH) is one of the most common dental conditions affecting most adults during their lifetime. Tubule occlusion is a widely accepted method for treating DH. Current in-vitro techniques such as focused ion beam, scanning electron microscopy (SEM), or hydraulic conductance that are used to determine tubule occlusion do not provide the depth of occlusion, are time-consuming, expensive and the volume of dentine tested is limited. The presented study aimed to assess the ability of serial block-face SEM (SBF-SEM) to section dentine, to quantify the number of occluded tubules including the depth of penetration by NovaMin and stannous fluoride (SnF2) and to compare mineral density between the control and treated dentine. Results demonstrated that NovaMin provided a better occlusion with 100% of the tubules blocked at the surface compared to 83% for SnF2. The grayscale value (230.42) was significantly higher (p <= 0.05) after treatment with NovaMin compared to SnF2(222.06) and the control (196.37), indicating increased mineral density and dentine mineralization. SBF-SEM has the potential to be used for large volume analysis of bone-like materials at high resolution with minimal sample preparation over a short period. It can be significantly useful in the development and research of new biomaterials.
机译：牙本质过敏症（DH）是影响大多数成年人一生的最常见的牙科疾病之一。肾小管阻塞是治疗DH的一种广泛接受的方法。目前用于确定小管阻塞的体外技术，如聚焦离子束、扫描电子显微镜（SEM）或水力传导，不提供阻塞深度，耗时、昂贵，且测试的牙本质体积有限。本研究旨在评估连续块面扫描电镜（SBF-SEM）切割牙本质的能力，量化堵塞小管的数量，包括诺瓦明和氟化亚锡（SnF2）的穿透深度，并比较对照牙本质和治疗牙本质之间的矿物密度。结果表明，与SnF2的83%相比，NovaMin在100%的小管表面阻塞的情况下提供了更好的阻塞。与SnF2（222.06）和对照组（196.37）相比，诺瓦明治疗后的灰度值（230.42）显著升高（p<=0.05），表明矿物质密度和牙本质矿化增加。SBF-SEM有可能在短时间内以最少的样品制备用于高分辨率的类骨材料的大体积分析。这对新型生物材料的开发和研究具有重要意义。

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