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首页> 外文期刊>Journal of Biophotonics >Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy
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Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging: Comparison with light and electron microscopy

机译：偏振分辨的SHG成像作为老化期间胶原蛋白改变的快速筛选方法：与光和电子显微镜的比较

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Our previous study on rat skin showed that cumulative oxidative pressure induces profound structural and ultrastructural alterations in both rat skin epidermis and dermis during aging. Here, we aimed to investigate the biophotonic properties of collagen as a main dermal component in the function of chronological aging. We used second harmonic generation (SHG) and two-photon excited fluorescence (TPEF) on 5 mu m thick skin paraffin sections from 15-day-, 1-month- and 21-month-old rats, respectively, to analyze collagen alterations, in comparison to conventional light and electron microscopy methods. Obtained results show that polarization-resolved SHG (PSHG) images can detect collagen fiber alterations in line with chronological aging and that this method is consistent with light and electron microscopy. Moreover, the beta coefficient calculated from PSHG images points out that delicate alterations lead to a more ordered structure of collagen molecules due to oxidative damage. The results of this study also open the possibility of successfully applying this fast and label-free method to previously fixed samples.
机译：我们之前对大鼠皮肤的研究表明，在衰老过程中，累积的氧化压力会导致大鼠皮肤表皮和真皮发生深刻的结构和超微结构改变。在这里，我们的目的是研究胶原蛋白的生物光子特性，它是皮肤衰老过程中的主要成分。我们分别在15天、1个月和21个月大鼠的5μm厚皮肤石蜡切片上使用二次谐波产生（SHG）和双光子激发荧光（TPEF）分析胶原变化，与传统的光学和电子显微镜方法进行比较。结果表明，偏振分辨SHG（PSHG）图像可以检测出胶原纤维随时间老化的变化，该方法与光学和电子显微镜一致。此外，根据PSHG图像计算出的β系数指出，由于氧化损伤，细微的改变会导致胶原分子结构更加有序。这项研究的结果也为成功地将这种快速且无标签的方法应用于之前固定的样本提供了可能性。

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