...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Biophotonics >Spectrophotometric evaluation of hemolysis in plasma by quantification of free oxyhemoglobin, methemoglobin, and methemalbumin in presence of bilirubin
【24h】

Spectrophotometric evaluation of hemolysis in plasma by quantification of free oxyhemoglobin, methemoglobin, and methemalbumin in presence of bilirubin

机译：通过定量游离氧合氟胺，甲基酚和甲状合磷蛋白在胆红素存在下血浆溶血分光光度法评价

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Severe intravascular hemolysis leads to the simultaneous presence of free heme pigments (oxyhemoglobin, methemoglobin, and methemalbumin) and bilirubin in human plasma. Standard spectrophotometric methods used to assess in vivo hemolysis inadequately address this complex analytical situation. Thus, we propose a novel quantification algorithm to ensure the highest analytical specificity. A corresponding second-derivative fitting algorithm was validated according to the guideline of bioanalytical method validation from the European Medicines Agency using plasma specimens (n = 1759) spiked with different concentrations of oxyhemoglobin and methemoglobin. The results were compared to standard spectrophotometric quantification methods described by Harboe, Noe, and Fairbanks. Based on the second-derivative method, simultaneous quantification of oxyhemoglobin and methemoglobin/methemalbumin in samples with total bilirubin concentrations <= 4.9 mg/dL (83.8 mu mol/L) provided robust results (inaccuracy <= 20%, imprecision <= 16%). Analyzing UV/VIS spectra of plasma from patients with confirmed severe intravascular hemolysis evidenced an underestimation of up to 33% for the combined free heme pigment content. The employed second-derivative algorithm allows for automated and highly specific quantification of the free heme pigment content in diluted human plasma, which cannot be realized with standard spectrophotometric evaluation methods. An Excel-based tool readily applicable to clinical datasets accompanies this manuscript.
机译：严重的血管内溶血导致人体血浆中同时存在游离血红素色素（氧合血红蛋白、高铁血红蛋白和高铁白蛋白）和胆红素。用于评估体内溶血的标准分光光度法不能充分解决这种复杂的分析情况。因此，我们提出了一种新的量化算法，以确保最高的分析特异性。根据欧洲药品管理局（European Medicines Agency，简称European Medicines Agency）的生物分析方法验证指南，使用添加了不同浓度氧合血红蛋白和高铁血红蛋白的血浆样本（n=1759），验证了相应的二阶导数拟合算法。结果与Harboe、Noe和Fairbanks描述的标准分光光度定量方法进行了比较。基于二阶导数法，在总胆红素浓度<=4.9 mg/dL（83.8μmol/L）的样品中同时定量氧合血红蛋白和高铁血红蛋白/高铁白蛋白，可提供稳健的结果（不准确度<=20%，不准确度<=16%）。对确诊为严重血管内溶血的患者血浆的紫外/可见光谱分析表明，对游离血红素色素的组合含量估计不足33%。所采用的二阶导数算法允许对稀释的人体血浆中的游离血红素色素含量进行自动化和高度特异性的定量，这是标准分光光度评估方法无法实现的。这篇手稿附带了一个基于Excel的工具，可以方便地应用于临床数据集。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号